Abstract: Nitrogen(N),phosphorus(P)and potassium(K)are important nutrients for flue-cured tobacco,which play a key role in the formation of yield and quality of the leaves.However,little research was focus on the effects of fertilization on content of aroma constituents in tobacco leaves.In the present paper,a field experiment was employed to study the effects of combining application of N,P and K fertilizers on chemical components and content of aroma constituents in flue-cured tobacco leaves using a tobacco(Nicotiana tabacum L.)cultivar K326.The results indicated that chemical components of tobacco leaves were not harmonious,and content of total aroma constituents and different kinds of aroma constituents were the lowest in the treatment of no fertilizer(CK).Treatments of application of single fertilizer of N,P,K or combining application of any two of them could improve the chemical components and increase the content of aroma constituents of cembranenoids and carotenoids.While the chemical components were more appropriate and the content of total aroma constituents and different kinds of aroma matter were higher in the treatment of combining application of N,P,K fertilizers compared with the other treatments.It suggested that balancing application of N,P,K fertilizers was the foundation for increasing the flavor quality of tobacco leaves.