Abstract: Soil salinity is an important limiting factor for agricultural production in arid and semiarid regions. The under-film drip irrigation has gained widespread popularity as an efficient and economically viable irrigation method for increasing water use efficiency and reducing the adverse effects of salinity on plant growth and yield. A pot experiment was carried out to investigate effects of soil salinity on nutrients and ions uptake in cotton under greenhouse conditions. Cotton was grown in plastic containers (height 54 cm, diameter 48 cm) filled with a loamy soil. Three soil salinity (EC1:5) level were set as 0.32, 1.12 and 1.90 dS/m（namely E1, EC2, EC3, respectively）. A drip irrigation system was used for water application. Dry matter accumulation of cotton was influenced significantly by soil salinity. Under higher salinity condition (EC1:5= 1.90 dS/m), Vegetative and reproductive growth of cotton were incoordinate and development was delayed. Shedding and seed cotton yield were decreased with salinity increase. Accumulations and distributions of N, P and K in cotton plant were affected by soil salinity. Accumulations of N, P and K in boll were markedly reduced by soil salinity, but there were no significant difference in leaf and stem among three treatments. With soil salinity increasing, content of Ca2+, Na+ and Cl- in cotton plant were increased. Ca2+, Na+ and Cl- were mainly accumulated in leaf and stem, but there were very low in seed cotton.