Abstract: High glucosinolate(GS) content represents the good quality of turnip, which is strongly affected by the nitrogen and sulfate nutrition of turnip. In this paper, the effects of different application levels of N (80 kg/ha, 160 kg/ha and 240 kg/ha, denoted by N1, N2 and N3) and S (10 kg/ha and 60 kg/ha, denoted by S1 and S2) on the composition and concentration of glucosinolate（GS）in turnip (cultivar: Baiyu) were studied using sand culture experiment and HPLC method. The results show that there are 7 kinds of glucosinolates in the shoots and 9 kinds of glucosinolates in the roots of Baiyu turnip. The total content of glucosinolates in turnip shoots is 1.16 times of that in roots. The glucosinolate contents in the roots and shoots are increased with the increase of sulfur supply, and the ratio of indole glucosinolate to total glucosinolate is significantly increased with the increase of the nitrogen supply. The highest glucosinolates content is obtained in the N2S2 treatment. Glucosinolates content of turnip dependent on the balance of nitrogen and sulfur supply.