• ISSN 1008-505X
  • CN 11-3996/S
LIU Yan-xia, LI Xiang, CAI Liu-ti, SHI Jun-xiong. Nursery application of a novel bioorganic fertilizer on controlling tobacco bacterial wilt[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(5): 1303-1313. DOI: 10.11674/zwyf.16368
Citation: LIU Yan-xia, LI Xiang, CAI Liu-ti, SHI Jun-xiong. Nursery application of a novel bioorganic fertilizer on controlling tobacco bacterial wilt[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(5): 1303-1313. DOI: 10.11674/zwyf.16368

Nursery application of a novel bioorganic fertilizer on controlling tobacco bacterial wilt

  • ObjectivesTobacco bacterial wilt is one of the most serious soil-borne diseases in tobacco growing area of Guizhou Province, China. Since bio-controlling tobacco bacterial wilt becomes more and more popular, it is of great importance to develop a high-efficient and economic way to bio-control this disease.
    MethodsIn this study, the antagonistic strain NJL-14 was isolated in our own lab, and a novel bioorganic fertilizer (BIO) was developed using the secondary solid-fermented approach. Nursery and pot experiments were conducted to evaluate efficiency of the BIO application on controlling tobacco bacterial wilt. In nursury substitute, 1%, 2%, 3%, 4% and 5% of BIO were added to test the suitable addition levles of BIO. In the follwing pot experiement, the addition level was used in the soil basal treatment. Using the seedlings from the nursury without treated with BIO, four treatments were set up as : healthy soil (un-infected with bacterial wilt), sick soil (infected with wilt), sick soil added with normal organic fertilizer , sick soil with BIO; and a treatment of using seedlings from BIO treated nursury and sick soil added with BIO. Real-time PCR and biology-ECO technology were employed to study population of bacteria and fungi in nursery substrate and soil, the soil microbial functional diversity, especially the effect on the reproduction of Ralstonia solanacearum and its antagonist.
    ResultsWhen the concentration of BIO was within 2%, there were no significant differences in the germination percentage between the control and the treatments. Compared with normal float seedlings, seedlings in tray stabilized the colonization of antagonistic strain NJL-14 in rhizosphere, and significantly increased tobacco seedling biomass. As for tobacco variety, K326, the dry weights of leaves and roots treated by BIO were increased by17.2% and 30.8% compared to the control. As for tobacco variety, Honghuadajinyuan, the dry weights of leaves and roots treated by BIO were 14.9% and 20.0% higher than that of the control. The best bio-control efficiency (100%) could be achieved by applying BIO at the nursery stage plus application in the transplanted soil, which was obviously better than the mode of only applying BIO in the pot experiment. Under the double-application mode, soil pathogen population was significantly depressed to less than 107 cfu/g soil. Meanwhile, the soil microbial functional diversity was improved a lot.
    ConclusionsThe mode of applying BIO at the nursery stage plus application in the transplanted soil could efficiently bio-control tobacco bacterial wilt, control soil pathogen concentration and improve soil microbial functional diversity. This work built a foundation for effectively bio-controlling tobacco bacterial wilt.
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