Effect of arbuscular mycorrhizal fungi inoculation on tobacco growth and arsenic pollution risk from phosphogypsum addition

【Objectives】 Arbuscular mycorrhizal fungi (AMF) could promote plant nutrients absorption and growth, and has a certain resistance to arsenic pollution in soil. Phosphogypsum (PG) could provide the necessary elements for crop growth because of containing nutrients such as phosphorus (P) and sulfur (S), but PG contains arsenic (As) which could bright pollution risk to crop growth. The objective of this study was to investigate the role of AMF inoculation on growth of tobacco cultivar, Yunyan 87, and the influence of Ascontamination risk from PG application. 【Methods】 A pot culture experiment was conducted to measure the plant biomass and P, S and As uptakes of tobacco under different PG application levels(0 and 40 g/kg)and different AMF species, none mycorrhizal(NM), Glomus mosseae(GM)and Glomus aggregatum(GA).【Results】The PG amendment significantly increases the content, total absorption amount and specific absorption rate of P in plants, and significantly increases the content and total absorption of S in tobacco shoots, and significantly increases tobacco shoot biomass with or without the inoculation of AMF. Except the NM treatment, the PG amendment significantly increases the tissue concentration, total absorption amount and specific absorption of S in tobacco roots, and significantly increases tobacco plant biomass. Compared with the NM treatment, the GM inoculationsignificantly increases the specific absorption rates of P and S in tobacco roots, and significantly increases the concentrations and total absorption amounts of P and S in “Yunyan 87” plantsPG application levelsin all. In addition, the GM inoculation significantly decreases the concentration and absorption of As in tobacco shoots, and significantly increases the absorption ratio of P to As in tobacco plants. Under the PG0 treatment, the GA inoculation significantly increases the plant concentration, total absorption of P, and significantly increases the tissue concentration, total absorption of S in tobacco shoots. Under the PG40 treatment, the GA inoculation significantly increases the tissue concentration, total absorption of S in tobacco roots, and significantly increases the tobacco plant biomass. 【Conclusion】 Regardless of PG application level, the GA inoculation decreases the tissue concentration and absorption amount of As in tobacco shoots at different degree, and thus increases the absorption ratio of P to As in tobacco shoots. Among all PG40 treatments, GM inoculation has the most desirable effect on tobacco growth, and could reduce the risk of As pollution caused by agricultural application of PG to some extent.