STUDY ON THE MECHANISM OF LATERAL BUD GROWTH OF BORON DEFICIENT PEA

The effect of boron deficiency on the growth and development of pea plants was studied using solution culture It was demonstrated that under the boron deficient condition the growth of pea plant was inhibited, and inhibition of the root growth appeared first(fig 1), 10 days after boron free treatment, the lateral buds was induced in lower part of the plant and the apical dominance was inhibited(fig 2) Another 14 days later, about 6 lateral buds were developed in the boron deficient plant, and the normal plants had no lateral buds At the treatment days of 0, 3, 6, 9, the apex buds (10cm length), the second and third shoot nodes (0.5cm length every node) and the roots were harvested for the measurement of the inner levels of IAA in Z/ZR dynamic with Enzyme Linked Immuno Sorbent Assay (ELISA) The level of IAA in shoot apex of boron deficient plant was higher than control(fig 3), but the diffusible IAA level in shoot apex(table 1) and the level of IAA in shoot node(fig 2) were lower under boron deficiency condition At the beginning of treatment (3～6 days), the levels of Z/ZR in root and shoot node of boron deficient plant were higher than that of boron deficient plant(fig 4) It is suggested that the lower level of IAA and the higher level of Z/ZR in shoot node of boron deficient is an important reason for lateral bud development