Objectives The biological properties and bacterial communities in the rhizosphere of canes infected and non-infected with ratoon stunting disease (RSD) were compared for effective bio-control of the RSD in sugarcanes.
Methods The cane plant and their rhizosphere soil were identified for infection of RSD firstly in lab. The cane plant and rhizosphere soil samples infected and non-infected by RSD were collected, respectively. The soil biological properties and bacterial community were determined by traditional and high throughput sequencing techniques.
Results Compared with healthy soil, the activities of β-glucosidase, phosphatase and aminopeptidase in RSD infected soils were significantly inhibited, and the contents of soil microbial biomass carbon, nitrogen and phosphorus were significantly decreased, and the indexes of bacterial diversity and richness including Chao1, Shannon were significantly decreased as well. As for individual bacteria community on phylum level, the ratios of Proteobacteria, Actinobacteria, Gemmatimonadetes and Nitrospirae were all significantly decreased, while those of Chloroflexi, Acidobacteria, Firmicutes, Cyanobacteria, Planctomycetes and Bacteroidetes were significantly increased in rhizospheres of RSD infected canes. On genus level, the ratios of Xanthobacteraceae, Acidothermus, Gaiellales, Roseiflexus, Micromonosporaceae and Nitrospira were significantly decreased, while those of Acidobacteria and unclassified bacteria were significantly increased in rhizospheres of RSD infected canes.
Conclusions In rhizosphere soils of RSD infected canes, the activities of important soil enzymes and microbial mass carbon, nitrogen and phosphorous contents are low, the diversity and the richness bacterial community are significantly decreased. On the phylum and genus levels, great variations in the abundance of the dominant bacteria have been confirmed.
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