Effect of calcium on key enzymes of nitrogen assimilation and related to protein phosphorylation in wheat

The mechanism of nitrogen assimilation on green wheat seedlings through adding different concentration of Ca2+ 、Ca2+ specific chelator EGTA and blocker of Ca2+ channel LaCl3 in nutrient solution (when treated use of EGTA or LaCl3, there was 1mmol/L Ca2+ in nutrient solution) was studied. The results showed that there was significant correlation between the key enzymes of nitrogen assimilation (nitrate reductase, NR; glutamine synthetase, GS) and Ca2+/CaM dependent protein kinase (Ca2+/CaM PK).It indicated that reversible protein phosphorylation could take an important part in regulating the signal transduction pathway of calcium.