氮素添加和干旱胁迫下白羊草碳氮磷化学计量特征

孙彩丽; 肖列; 李鹏; 薛萐; 刘国彬

doi:10.11674/zwyf.17006

氮素添加和干旱胁迫下白羊草碳氮磷化学计量特征

孙彩丽¹,
肖列²,
李鹏²,
薛萐^1,3,
刘国彬^1,3,*, ,

1.
西北农林科技大学水土保持研究所黄土高原土壤侵蚀与旱地农业国家重点实验室, 陕西杨凌 712100;
2.
西安理工大学西北旱区生态水利工程国家重点实验室培育基地, 陕西西安 710048;
3.
中国科学院水利部水土保持研究所, 陕西杨凌 712100

基金项目:

中科院西部青年学者项目（XAB2015A05）

国家自然科学基金项目（41371510，41371508，41471438）资助。

详细信息

作者简介:
孙彩丽(1989-),女,河南平顶山人,博士,主要从事水土保持及土壤微生物生态方面研究。E-mail:suncaili2007@126.com

通讯作者:
刘国彬

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出版历程
- 收稿日期: 2017-08-02
- 修回日期: 2017-08-02
- 刊出日期: 2017-07-24

Effects of nitrogen addition and drought stress on carbon, nitrogen and phosphorus stoichiometry of Bothriochloa ischaemum

SUN Cai-li¹,
XIAO Lie²,
LI Peng²,
XUE Sha^1,3,
LIU Guo-bin^1,3,*, ,

1.
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Northwest AF University, Yangling, Shaanxi 712100, China;
2.
State Key Laboratory Base of Eco-hydraulic Engineering in Arid Area, Xi'an University of Technology, Xi'an 710048, China;
3.
Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resource, Yangling, Shaanxi 712100, China

摘要

摘要: [目的]氮素和水分是干旱半干旱区生态系统的主要限制因子，研究两者交互作用对干旱半干旱区植物碳（C）、氮（N）、磷（P）化学计量特征的影响有助于深入了解干旱半干旱生态系统对全球变化的响应。[方法]以黄土丘陵区退耕地典型草本植物白羊草（Bothriochloa ischaemum）为研究对象，采用盆栽控制试验，设置添加氮0（对照）、2.5 g/（m2a）（低氮）、5.0 g/（m2a）（高氮）三个水平；供水处理设75%~80% FC（充分供水）、55%~60% FC（轻度干旱胁迫）和35%~40% FC（重度干旱胁迫）三个水平。测定了白羊草地上部分和根系碳氮磷含量，讨论了氮素和水分供应对其化学计量特征的影响。[结果]氮素添加和干旱胁迫对白羊草地上部分和根系碳含量无显著影响，氮素添加使白羊草地上部分氮含量提高9.7%~48.8%（P 0.001），而干旱胁迫使其降低2.8%~28.3%（P 0.001）。氮素添加和干旱胁迫对白羊草根系氮含量的影响表现为正常水分条件下氮素添加使根系氮含量提高25.0%~26.1%（P 0.01），而干旱条件下氮素添加无显著作用。氮素添加和干旱胁迫使白羊草地上部分磷含量分别降低17.4%~31.8%和12.0%~22.1%（P 0.001）。氮素添加和干旱胁迫对白羊草地上部分C：N的影响表现为在干旱胁迫条件下氮素添加使地上部分C：N降低24.9%~32.9%（P 0.05），在正常供水条件下氮素添加无显著影响。氮素添加对根系部分C：N有显著影响，在正常供水条件下氮素添加使根系部分C：N降低19.8%~24.5%（P 0.05）。氮素添加和干旱胁迫使白羊草地上部分C：P分别提高24.4%~42.3%和12.2%~31.0%（P 0.001），对根系C：P无显著影响。氮素添加显著提高白羊草地上部分N：P，干旱胁迫对白羊草地上部分N：P无显著影响。氮素添加和干旱胁迫对白羊草根系部分N：P表现为在正常供水条件下氮素添加使根系部分N：P提高26.8%~54.8%（P 0.05），在干旱胁迫条件下氮素添加无显著影响。[结论]氮素添加条件下白羊草C：P和N：P的提高表明氮沉降增加一定程度上改善了土壤供氮状况，进一步加剧了磷素限制作用。氮素增加条件下干旱胁迫对N：P无显著影响，表明白羊草的生长将逐渐受到氮素和磷素的共同限制。
- 白羊草 /
- 化学计量学 /
- 干旱 /
- 氮素添加 /
- 养分限制 /
- 黄土高原
Abstract: [Objectives] Both nitrogen and water shortage are the main limiting factors for plant growth in arid and semiarid ecosystems. Study the carbon (C), nitrogen (N) and phosphorus (P) stoichiometric characteristics of plant under nitrogen deposition and drought stress will help understanding the response characteristics of arid and semiarid ecosystems to global changes. [Methods] A pot experiment was conducted inside a phytotron using Bothriochloa ischaemum as tested martials. N application rates of 0, 2.5 and 5.0 g/(m2a) were designed, representing CK, low N and high N rate; and water supply levels of field capacity of 75%-80%, 55%-60% and 35%-40% were setup, representing drought stress level of well-watered, moderate drought stress and severe drought stress in turn. The contents of C, N and P in the shoot and root were determined and the C, N, and P stoichiometry were calculated. [Results] N addition and drought stress had no significant influence on C concentration in the aboveground and root of B. ischaemum. N addition increased the N concentration by 9.7%-48.8% (P 0.001) in the shoot, while drought stress decreased it by 2.8%-28.3% (P 0.001). Nitrogen addition and drought stress had significant interactive effects on the root N concentration, N addition increased the N concentration by 25.0%-26.1% (P 0.01) under well-watered condition and had no significant influence on it under drought stress condition. N addition and drought stress significantly decreased the P concentration by 17.4%-31.8% and 12.0%-22.1% in shoot. N addition and drought stress had significant interactive effects on the C:N ratio in shoot, N addition increased the C:N ratio by 24.9%-32.9% (P 0.05) under drought stress, but not under well-watered condition. The C:N ratio in the root of B. ischaemum was decreased by 19.8%-24.5% (P 0.05) by N addition under well watered condition. N addition and drought stress decreased the C:P ratio by 24.4%-42.3% and 12.2%-31.0% in the shoot of B. ischaemum, respectively, but not in the root. N addition significantly increased the N:P ratio in the aboveground part of B. ischaemum, drought stress did not. N addition and drought stress had significant interactive influence on the N:P ratio in the root of B. ischaemum, N addition increased the N:P ratio by 26.8%-54.8% (P 0.05) under well-watered condition and had no significant influence on it under drought stress condition.[Conclusions] N addition will increase the C:P and N:P ratio in the aboveground part of B. ischaemum under drought stress, intimating that the growth of B. ischaemum would be restrained by both N and P on the Loess Plateau with the aggravation of drought stress and N deposition to some extent.
- Bothriochloa ischaemum /
- stoichiometry /
- drought /
- nitrogen addition /
- nutrient limitation /
- Loess Plateau

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