15N2还原法测定胡萝卜离体类根瘤的固氮作用
NITROGEN FIXATION OF PARA-NODULES OF ORGAN CULTURED CARROT ROOTS WITH 15N2 REDUCTION METHOD
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摘要: 用低浓度的2,4-D处理胡萝卜离体根诱导结瘤,并接种固氮螺菌(Azospirillum.brasilense),用15N2还原法测定结果证明,胡萝卜离体类根瘤能固定15N2。电镜切片观察发现,类根瘤内有菌体存在,菌体进入细胞间隙中比进入细胞内多。Abstract: Nitrogen fixation of para-nodules of organ cultured carrot roots was assured with 15N2 reduction method after the par a-nodules of carrot roots were induced with low concentration 2,4-D and the inoculation of Azospirillum brasilense. More bacteria in intercellular spaces and less in cells of para-nodules were also observed under electronic microscope.
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