Abstract: Three compositae plants, as Calendula officinalis, Tagetes patula, Tagetes erecta, were cultured in N, P, K, Ca, Mg and S deficient solution. The nutrient deficiency symptoms, biomass and nutrient concentration of plant were studied. The results showed that N deficiency of Calendula officinalis and Tagetes erecta were most visible, while the deficiency of Tagetes patula cultured under K deprived solution was most visible. Compared to CK, N concentration of Calendula officinalis and Tagetes erecta increased when cultured under K deprived solution. Of all the three plants, P concentration increased when plant grown without N or K, and K concentration decreased when P was deficient. K and Mg concentration increased when Ca was deficient. Ca concentration was increased when K was not supplied. K concentration of Calendula officinalis was enhanced when Mg was not supplied. S concentration of three plans was not decreased gradually without S being supplied, and S concentration of Tagetes patula and Tagetes erecta were enhanced when N, P, K and Ca were deficient.