Abstract: A 4 years organic and inorganic N combined application experiment was conducted to study the effects of N input types and rates on the grain yield and quality of different winter wheat cultivars. Three N input types(organic N, inorganic N, organic N combined with inorganic N), six N input rates(N 0, 45, 90, 120, 180, 240 kg/ha) and two winter wheat cultivars(Shimai 15 and Weimai 8) were selected in this paper. The results showed that cultivars, N input types and rates had significant effects on grain yield and quality parameters. The grain yield, sedimentation value, development time, stability time of Shimai 15 were 12.62%, 5.09%, 5.85%, 25.35% higher than those of Weimai 8, crude protein, wet gluten and water absorption of Shimai 15 were 11.03%, 15.51%, 5.49% lower than those of Weimai 8. The grain yield, crude protein, wet gluten, sedimentation value and development time were significantly positive correlated with plant N uptake, while the water absorption and stability time had low correlations with plant N uptake. The treatments of 0/180 and 0/240(inorganic N 180 and 240 kg/ha) had the highest plant N uptakes and grain yields and improved grain quality which were also true in the treatment of 120/120(combined organic N 120 kg/ha with inorganic N 120 kg/ha). When organic N was single applied, all the high(240 kg/ha), middle(120 kg/ha) and low(45 kg/ha) N input rates had the lowest plant N uptake and grain yields and quality compared to the same N input rate of inorganic N single application and organic and inorganic N combined application due to the low nitrogen supply capacity of manure.