• ISSN 1008-505X
  • CN 11-3996/S

含有益生细菌成分的种子处理剂浸种对食用百合根际土壤微生物区系的调控效应

Seed treating agent containing Beneficial Microbial Regulated rhizosphere soil Microorganism Structure of Lanzhou lily

  • 摘要:
    目的 播种前应用有益微生物浸种是兰州百合(Lilium davidii var. unicolor)栽培中尚未应用的一项新技术。我们利用新一代测序技术(NGS)研究这种浸种方法对真菌和细菌群落结构的影响。
    方法 将百合鳞茎浸泡在一种含有有益微生物的种子处理剂(SP处理)中4小时,然后于7月种植在土壤中,并于9月取样调查植物生长情况、根际层土壤理化性质和微生物群落结构。此外,我们还利用 PICRUSt 和 FUNGuild 软件预测了细菌通路和真菌功能。
    结果 在 SP 处理下,真菌和细菌群落结构发生了显著变化,同时土壤养分条件也得到了改善。值得注意的是,优势微生物群落的相对丰度发生了显著变化,如真菌BasidiomycotaPseudeurotiumCladophialophoraMicroascusDactylonectria;以及细菌ProteobacteriaChloroflexiOchrobactriumLysobacterRB41。微生物功能预测结果表明,在 SP 处理下,病原真菌(包括植物病原体)减少,内生真菌和腐生真菌增加。在前 20 种代谢途径中,与 CK 相比,80% 在 SP 处理中上调。
    结论 用有益微生物浸种能促进兰州百合球茎的生长。有益微生物在调节土壤微生物结构、增强内生真菌积累、减少病原体和改善土壤功能方面发挥了重要作用。此外,研究还发现特定的微生物群参与了土壤健康的维护。

     

    Abstract:
    Background The application of beneficial microbial seed soaking prior to sowing represents a novel technology that has yet to be employed in Lanzhou lily cultivation. We conducted an experiment to investigate the impact of this soaking method on the fungal and bacterial community structures using next-generation sequencing technology (NGS).
    Methods Lily bulbs were soaked in a kind of seed treating agent containing beneficial microbial (SP treatment) for 4 hours, then planted in soil in July and sampled in September for investigation of the plant growth, and the rhizosphere soil physicochemical properties and the microorganism community structures. Furthermore, we utilized the software PICRUSt and FUNGuild to predict bacterial pathways and fungal functions.
    Results Under SP treatment, there were significant alterations in fungi and bacteria community structures, companied by improved soil nutrient conditions. Nota, the relative abundance of dominant microorganism groups, such as the fungi Basidiomycota, Pseudeurotium, Cladophialophora, Microascus, and Dactylonectria; as well as the bacterial Proteobacteria, Chloroflexi and Ochrobactrium, Lysobacter and RB41, underwent notable changes. Microorganism function prediction results indicated a reduction in pathotrophic fungi (including the plant pathogens) and an increase in endophytic and saprotrophic fungi under SP treatment. Among the top 20 metabolism pathways, 80% were upregulated in SP treatments compared to the CK.
    Conclusions Seed soaking with beneficial microbial strain promoted growth of Lanzhou lily bulbs. The beneficial microorganisms played a crucial role in regulating soil microbial structures, enhancing the accumulation of endophytic fungi, reducing pathogens, and improving soil function. Furthermore, specific microbial groups were fund to be involved in maintaining soil health.

     

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