• ISSN 1008-505X
  • CN 11-3996/S

光氮互作对杉木幼苗抗氧化能力及内源激素水平的影响

Effects of light and nitrogen interaction on antioxidant capacity and endogenous hormones of Cunninghamia lanceolata seedlings

  • 摘要:
    目的 适宜的光照强度和氮素供应水平是提高苗木质量的关键因子,为此,本研究分析了不同光强和氮素营养下杉木(Cunninghamia lanceolata)幼苗的生长状况及主要生理指标。
    方法 以1年生杉木幼苗为材料,采用双因素随机区组试验设计,在福建顺昌县国有林场苗圃地进行盆栽试验。设置4个自然光照强度:100% (L0)、75% (L1)、45% (L2)、20% (L3),3个氮素水平:N0 (0 g/株)、N1 (4 g/株)和N2 (6 g/株)。处理1年后,测定杉木幼苗苗高、地径、抗性生理和内源激素指标,结合隶属函数法筛选适合杉木幼苗生长的光氮组合处理。
    结果 与L0N0相比,适当遮荫促进杉木苗高和地径生长,施氮能促进杉木苗高和地径的生长,最佳处理为L2N1,其苗高和地径增量较L0N0分别增加了139.54%和89.84%,其次为L2N2。遮荫和施氮不同程度提高了杉木叶片过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性和总抗氧化能力,降低了过氧化氢(H2O2)和丙二醛(MDA)含量。CAT、SOD、POD活性和总抗氧化能力均以L2N1、L2N2处理较高,L2N1处理的CAT、SOD、POD活性和总抗氧化能力较L0N0分别提高131.67%、24.26%、625.70%和141.84%,H2O2和MDA含量则分别下降了72.71%和66.42%。遮荫降低杉木顶芽细胞分裂素 (CTK)、脱落酸 (ABA)和茎中赤霉素 (GA)、ABA含量,但可提高CTK/ABA、GA/ABA、IAA (吲哚乙酸) /ABA值。不同遮荫处理下,施氮均显著提高了杉木顶芽和茎中IAA、GA、ABA含量,以及CTK/GA、GA/ABA、IAA/ABA值(P<0.01),且施氮后不同遮荫处理的激素水平和比值没有显著变化。隶属函数分析结果表明,最有利于杉木幼苗生长的光氮组合处理为L2N1处理。
    结论 适宜的光氮组合能有效提升杉木幼苗芽中的IAA和GA含量,提高IAA/ABA和GA/ABA值,增强叶片中的抗氧化酶活性和幼苗抗逆能力,促进幼苗株高和地径生长,从而培育出优良的种苗。供试土壤和气候条件下,最有利于一年生杉木幼苗生长的遮荫率为45% 光强542.68 μmol/(m2·s)、氮肥施用量N 4 g/株。

     

    Abstract:
    Objectives Light intensity and nitrogen supply heavily affect the growth of Cunninghamia lanceolata seedlings. We measured the growth and physiological indices of Cunninghamia lanceolata seedlings under different light intensity and nitrogen application rates.
    Methods A two-factor randomized block design pot experiment was conducted in the state owned Cunninghamia lanceolata seedling forest, located in Shunchang City, Fujian Province, one-year-old seedlings was used as test materials. Four shading levels were applied to simulate natural light reduction: 0% shading (L0, full sunlight), 25% shading (L1), 55% shading (L2), and 80% shading (L3). Three N levels were administered per seedling: N 0 g (N0, control), N 4 g (N1, moderate), and N 6 g (N2, high). The seedlings were exposed to the experimental conditions for one year, with regular maintenance to ensure consistent growth conditions. Then seedling height and ground diameter were measured, the antioxidant indices in leaves were analyzed to calculate the resistance to stress, and the endogenous hormone contents in apical buds were measured as well. The promotion effect of treatments was screened by subordination function method.
    Results Compared with L0N0, suitable shading promoted the height and ground diameter growth of C. lanceolata seedlings, nitrogen application further enhanced the promoting effect of shading. L2N1 was recorded the most suitable light-nitrogen combination, with seedling height and ground diameter increased by 139.54% and 89.84%, compared to L0N0. Both shading and nitrogen application increased the activities of peroxidase (POD), catalase (CAT), and superoxide dismutase (SOD), reduced the accumulation of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in the leaves C. lanceolata seedlings, thereby enhancing the seedlings’ total antioxidant capacity. L2N1 and L2N2 were tested higher CAT, SOD, POD activities and total antioxidant capacities, with the increment in L2N1 by 131.67%, 24.26%, 625.70%, and 141.84%, and decline in H2O2 and MDA contents by 72.71% and 66.42% as compared with L0N0, respectively. Shading led to lower cytokinin (CTK) and abscisic acid (ABA) content in the buds and gibberellic acid (GA) and ABA content in the stems of C. lanceolata, but higher ratios of CTK/ABA, GA/ABA, and IAA (indoleacetic acid) /ABA. N application offset the inhibition of hormones by shading, and significantly improved the contents of IAA, GA and ABA, as well as the ratios of CTK/GA, GA/ABA and IAA/ABA in buds and stems of C. lanceolata seedlings (P<0.01), and the effect was not significantly different between N1 and N2. The subordination function analysis results showed that the most suitable light-nitrogen combination treatment for the growth of C. lanceolata seedlings was L2N1 treatment.
    Conclusions An appropriate light-nitrogen combination can effectively increase the IAA and GA contents and the IAA/ABA and GA/ABA ratios, decrease the CTK/GA ratio in buds, and enhance the antioxidant enzyme activity, which results in high scavenging capacity, as a consequencece promoting the growth of C. lanceolata seedlings. Under the test conditions, the suitable light intensity and nitrogen application rate for high quality C. lanceolata seedling production are 542.68 μmol/(m2·s) and 4 g/seedling, respectively.

     

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