• ISSN 1008-505X
  • CN 11-3996/S

深翻与适量氮肥协同提升旱地小麦花后水氮利用与光合性能

Deep plowing and optimal nitrogen fertilization synergistically enhance post-anthesis water and nitrogen utilization and photosynthetic performance

  • 摘要:
    目的 旱地麦田在花后阶段容易出现干旱和脱肥现象,是导致减产的重要原因。研究蓄水和施肥措施对水肥资源高效利用及产量提升的调控机制,对实现旱地小麦稳产增产意义重大。
    方法 于2022—2024年在山西闻喜旱地小麦试验基地开展定位试验,研究不同降水年型下耕作方式(免耕和深翻)和氮肥施用量(135、180和225 kg/hm2)对旱地小麦花后土壤水分消耗、光合特性、氮素转移利用及产量的影响。
    结果 深翻较免耕显著增加开花期0—200 cm土层土壤蓄水量,湿润年增加4.43~8.39 mm,干旱年增加10.74~28.63 mm,且深层100—200 cm土层蓄水效应更显著。深翻较免耕促进花后土壤耗水向深层转移,湿润年配施225 kg/hm2氮肥较其他氮肥处理耗水量提高11.35 mm,干旱年配施135 kg/hm2氮肥较其他氮肥处理耗水增加36.33~43.48 mm。深翻较免耕显著提高旗叶核酮糖-1,5-二磷酸羧化酶/加氧酶 (ribulose-1,5-bisphosphate carboxylase/oxygenase,Rubisco)与Rubisco 活化酶 (Rubisco activase,RCA)活性峰值(花后25天);湿润年深翻配施氮225 kg/hm2与干旱年深翻或免耕配施氮135 kg/hm2均显著提升花后净光合速率。深翻较免耕增加花前氮素转运量,并协同花后深层耗水驱动氮积累,干旱年配施135 kg/hm2氮肥处理花后氮积累量及其对籽粒贡献率较其他处理分别提高72.65%~89.03%和24.91个百分点~31.32个百分点。产量与水氮效率分析表明,深翻显著提高单位面积穗数、穗粒数和千粒重,从而提高产量,其中湿润年增产18.87%~22.63%,干旱年增产9.74%~29.70%;湿润年配施225 kg/hm2氮肥处理通过提高单位面积穗数3.16%~12.25%和千粒重1.29%~5.17%,实现最高产量8014.69 kg/hm2,但水氮利用效率下降;干旱年配施180 kg/hm2氮肥处理获得最高产量6168.78 kg/hm2,提高水氮利用效率,其中产量和水分利用效率与施氮135 kg/hm2处理差异不显著。相关性分析结果证实花后Rubisco活性、茎秆氮运转量与深层耗水显著正相关,构成产量提升的生理基础。
    结论 休闲期深翻结合适宜施氮量(湿润年施氮180~225 kg/hm2;干旱年施氮135~180 kg/hm2)利于蓄积降水并保存于100 cm土层以下,供给小麦花后阶段消耗,强化花后水氮利用,驱动光合性能提高和产量形成,增强雨养农业生态系统的气候韧性,为旱地小麦高产高效栽培提供理论依据。

     

    Abstract:
    Objectives Post-anthesis drought and nutrient depletion are major constraints on yield formation in dryland wheat systems. Optimizing soil moisture conservation and fertilization strategies is therefore imperative for enhancing the synergistic efficiency of water and nitrogen utilization and improving grain yield.
    Methods A field experiment was conducted from 2022 to 2024 at the dryland wheat experimental base in Wenxi, Shanxi Province, China. Treatments consisted of two tillage practices (no-tillage and deep plowing) combined with three nitrogen fertilizer application rates (135, 180, and 225 kg/hm2) under different precipitation year conditions. Post-anthesis soil moisture consumption, photosynthetic characteristics, nitrogen translocation and utilization, and yield of dryland wheat were systematically evaluated.
    Results Compared with no-tillage, deep plowing during the fallow period significantly increased soil water storage in the 0−200 cm layer at anthesis, with increases of 4.43−8.39 mm in wet years and 10.74−28.63 mm in dry years, mainly attributable to enhanced water storage in the deep layer (100−200 cm) . Compared to no tillage, deep plowing promoted post-anthesis soil water consumption toward deeper layers. In wet years, deep plowing combined with 225 kg/hm2 nitrogen fertilizer compared to other nitrogen fertilizer treatments, increased post-anthesis water consumption by 11.35 mm, whereas in dry years, deep plowing combined with 135 kg/hm2 nitrogen fertilizer compared to other nitrogen fertilizer treatments increased water consumption by 36.33−43.48 mm. Compared to no tillage, deep plowing during the fallow period significantly increased the peak activities of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase (RCA) in flag leaves at 25 days after anthesis. Correspondingly the post-anthesis net photosynthetic rate increased in wet years under deep plowing combined with nitrogen fertilizer of 225 kg/hm2 and in dry years under deep plowing or no tillage combined with nitrogen fertilizer of 135 kg/hm2. Moreover, compared to no tillage, deep plowing during the fallow period increased pre-anthesis nitrogen translocation and in synergy with increased post-anthesis deep-layer water consumption, promoted nitrogen accumulation. Compared with other treatments, the treatment of 135 kg/hm² nitrogen fertilizer in dry year, increased post-anthesis nitrogen accumulation 72.65%−89.03% and percentage points of 24.91−31.32. Yield and water-nitrogen use efficiency analyses showed that deep plowing during the fallow period significantly increased spike number perarea, grains per spike, and 1000-grain weight, thereby resulting in yield increases of 18.87%−22.63% in wet year and 9.74%−29.70% in dry year. In wet years, the 225 kg/hm2 nitrogen fertilizer treatment achieved the highest yield of 8014.69 kg/hm2 by increasing spike number perarea by 3.16%−12.25% and 1000-grain weight by 1.29%−5.17%, but reduced water and nitrogen use efficiency. In dry years, the 180 kg/hm2 nitrogen fertilizer treatment obtained the highest yield of 6168.78 kg/hm2 and improved water and nitrogen use efficiency, with no significant differences in yield and water use efficiency compared with the 135 kg/hm2 treatment. Correlation analysis revealed significant positive relationship among post-anthesis Rubisco activity, stem nitrogen translocation, and deep-layer water consumption, which constituted the physiological basis for yield improvement.
    Conclusions Deep plowing during the fallow period combined with appropriate nitrogen application rates (180−225 kg/hm2 in wet years and 135−180 kg/hm2 in dry years) facilitates precipitation storage in soil layers below 100 cm and its subsequent utilization after anthesis. This management strategy strengthens post-anthesis water and nitrogen use, improves photosynthetic performance and yield formation, and enhances the climate resilience of rain-fed agricultural ecosystems. these findings provide a theoretical basis for high-yield and high-efficiency cultivation of dryland wheat.

     

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