Abstract: Planting density is an important practice for cotton production. In order to study the effects of different planting densities on nutrition uptake and yield of transgenic cotton cultivar, Xiangzamian 8, a field experiment was conducted by using six planting densities, 12000, 21000, 30000, 39000, 48000 and 57000 plant/ha, under high yield cultivated conditions in Jiangsu. The results show that the accumulative amounts of N, P and K in cotton plants are increased when the planting density is increased, while the N, P and K accumulative amounts and nutrient economic coefficients in the reproductive organs are in parabola functions, and the N, P and K accumulative amounts and nutrient economic coefficients in the reproductive organ are the highest under the 30000 plant/ha. In addition, the highest lint yield is 1610.3 kg/ha under the 30000 plant/ha. The percentages of N, P and K uptakes of cotton are the highest from the initial flowering stage to the peak flowering stage during the whole development stages, and the percentages of N, P and K uptakes from the initial flowering stage to the peak flowering stage are reduced when the planting density is increased. With the increase of plant density, the nutrient uptakes per 100 kg lint are increased, but the percentage of K uptake is reduced. Increasing the planting density reduces N, P, and K contents and enhances N, P, and K accumulations per plant in different fruit branches, but accelerates branch senescence, especially the lower branches. These results are consistent with the weights of bolls in different fruit branches.