• ISSN 1008-505X
  • CN 11-3996/S
闵炜芳, 方晶莹, 石亚飞, 摆小蓉, 舍杨梦斐, 田浩天, 罗成科. 硝普钠添加对碱胁迫下萌发期水稻碳氮代谢的影响[J]. 植物营养与肥料学报. DOI: 10.11674/zwyf.2023555
引用本文: 闵炜芳, 方晶莹, 石亚飞, 摆小蓉, 舍杨梦斐, 田浩天, 罗成科. 硝普钠添加对碱胁迫下萌发期水稻碳氮代谢的影响[J]. 植物营养与肥料学报. DOI: 10.11674/zwyf.2023555
MIN Wei-fang, FANG jing-ying, SHI Ya-fei, BAI Xiao-rong, SHE Yangmeng-fei, TIAN Hao-tian, LUO Cheng-ke. Effect of sodium nitroprusside addition on carbon and nitrogen metabolism of rice during germination under alkali stress[J]. Journal of Plant Nutrition and Fertilizers. DOI: 10.11674/zwyf.2023555
Citation: MIN Wei-fang, FANG jing-ying, SHI Ya-fei, BAI Xiao-rong, SHE Yangmeng-fei, TIAN Hao-tian, LUO Cheng-ke. Effect of sodium nitroprusside addition on carbon and nitrogen metabolism of rice during germination under alkali stress[J]. Journal of Plant Nutrition and Fertilizers. DOI: 10.11674/zwyf.2023555

硝普钠添加对碱胁迫下萌发期水稻碳氮代谢的影响

Effect of sodium nitroprusside addition on carbon and nitrogen metabolism of rice during germination under alkali stress

  • 摘要:
    目的 施加外源硝普纳 (sodium nitroprusside, SNP)有效缓解水稻碱胁迫伤害。本研究通过比较碱敏感差异水稻品种萌发及碳氮代谢相关指标的差异,探讨碱胁迫下外源SNP添加对萌发期水稻碳氮代谢的影响,为盐碱地水稻种植提供一定的参考。
    方法 以碱敏感品种中花11 (ZH11)和耐碱品种宁粳52 (NG52)为试验材料,混合碱胁迫 (alkali stress (AS), 20 mmol/L, CO32−和HCO3, pH 10.50)下添加不同SNP浓度 (0、10、30、50、70、100 μmol/L)浸种处理,通过测定萌发期关键生长指标,以筛选SNP最适浓度。结果显示碱胁迫下不同浓度外源SNP影响了水稻萌发,其中,30 μmol/L SNP处理显著增加了水稻的发芽指数,促进了水稻萌发。进一步设置CK (control)、SNP (30 μmol/L)、AS (20 mmol/L)、AS+SNP (20 mmol/L AS + 30 μmol/L SNP) 4个处理分析它们对萌发期碱敏感差异水稻碳氮代谢的影响。
    结果  (1) 在碳代谢方面,与AS处理相比,AS+SNP处理下ZH11和NG52中蔗糖含量显著降低。其中,NG52中蔗糖含量显著低于ZH11,而其蔗糖合成基因 (OsNIN1)和葡萄糖合成基因 (OsSPS1、OsSPS11)的表达量显著高于ZH11。 (2)在氮代谢方面,与AS处理相比,AS+SNP处理显著增加了2个品种中苹果酸、柠檬酸、一氧化氮 (nitric oxide, NO)含量,硝酸还原酶 (Nitrate Reductase, NR)、S-亚基谷胱甘肽还原酶 (S-nitrosoglutathione reductase, GSNOR)活性以及OsNR2OsGSNOR1基因表达,降低了NO3和氧化型谷胱甘肽 (Glutathione Oxidized, GSSG)含量,提高了脯氨酸 (Proline, Pro)水平。其中,NG52中苹果酸、柠檬酸、一氧化氮、脯氨酸、氧化型谷胱甘肽含量,S-亚基谷胱甘肽还原酶活性以及OsNR1.2、OsGSNOR1的表达量均显著高于ZH11。 (3)相关性和主成分分析葡萄糖和蔗糖合成酶基因 (OsNIN1)分别与苹果酸、柠檬酸、脯氨酸和一氧化氮呈显著正相关,葡萄糖、果糖、一氧化氮、亚硝基谷胱甘肽 (S-Nitroso-L-glutathione,GSNO)和氧化型谷胱甘肽可能是影响碳氮代谢相互协调的关键指标。
    结论 施加30 μmol/L SNP促进碱胁迫下水稻中蔗糖分解和有机酸的积累,增强了硝酸还原酶和S-亚基谷胱甘肽还原酶酶活性,提高了有机氮物质含量,促使碳代谢向氮代谢转化,维持了碱胁迫下萌发期水稻碳氮代谢的正常进行。

     

    Abstract:
    Objectives The application of exogenous sodium nitroprusside (SNP) can effectively alleviated alkali stress-induced damage in rice. This study aimed to elucidate the effects of exogenous SNP treatment on the carbon and nitrogen metabolism of rice cultivars different in alkali stress resistance during germination.
    Methods This study used alkali sensitive Zhonghua 11(ZH11) and alkali resistant Ninggeng 52(NG52) rice varieties. To determine the optimal SNP concentration, key growth indices during germination were measured by treating rice seed with different SNP concentrations (10, 30, 50, 70, 100 μmol/L) under alkali stress (AS, 20 mmol/L, CO32− and HCO3, pH 10.50). Furthermore, the effect of four treatments, including CK (control), SNP (30 μmol/L), AS (20 mmol/L), and AS+SNP (20 mmol/L AS + 30 μmol/L SNP) on carbon and nitrogen metabolism in different alkali-sensitive rice at germination stage were assessed.
    Results (1) Carbon metabolism Exogenous SNP concentrations affected rice germination under alkali stress, applying 30 μmol/L SNP significantly increased the germination index of rice. Moreover, the sucrose content in cultivar ZH11 and NG52 was significantly reduced after exposure to AS+SNP treatment compared with AS treatment. The sucrose contents in NG52 were significantly lower than those in ZH11, however, the expression of genes involved in sucrose synthesis (OsNIN1) and glucose synthesis (OsSPS1 and OsSPS11) were significantly higher in NG52 than that in ZH11. (2) Nitrogen metabolism compared with AS, the AS+SNP treatment markedly increased the levels of malic acid, citric acid and nitric oxide (NO), as well as the activities of nitrate reductase (NR) and S-nitroso glutathione reductase (GSNOR) and expression of OsNR2 and OsGSNOR1. However, the levels of NO3 and glutathione (oxidized glutathione, GSSG) were decreased, while that of proline (Pro) was increased in both varieties. Furthermore, the contents of malic acid, citric acid, NO, Pro, GSSG, together with GSNOR activity and the expression levels of OsNR1.2 and OsGSNOR1 were significantly higher in NG52 compared with ZH11. (3) Correlation analysis Significant positive correlations was existed between genes involved in glucose and sucrose synthesis (OsNIN1) with malic acid, citric acid, Pro, and NO. The principal component analysis indicated that glucose, fructose, NO, GSNO, and GSSG were key factors affecting the coordination of carbon and nitrogen metabolism.
    Conclusions Foliar application of 30 μmol/L SNP is effective in increasing sucrose breakdown, the accumulation of organic acids, enzyme activities of NR and GSNOR, organic nitrogen levels, and the transformation of carbon to nitrogen metabolism, as well as maintaining normal carbon and nitrogen metabolism of rice under alkali stress during germination.

     

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