- ISSN 1008-505X
- CN 11-3996/S
| Citation: |
LIANG Zhen-kai, GUO Cong-ying, WANG Cai-zhi, LI You-jun, ZHANG Jun. Synergistic effect of combined application of nitrogen and zinc on construction of good morphology and high physiological activities of wheat root[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(5): 826-839. DOI: 10.11674/zwyf.19286
|
Both nitrogen and zinc are essential nutrients for the growth, yield and quality of crops. We studied the root morphology and physiological characteristics of wheat under combined application of nitrogen and zinc, to deeply understand the mechanism of the yield and quality effects of N and Zn application.
Field experiments were conducted in the farm of Henan University of Science and Technology from 2016 to 2018, using wheat cultivar of ‘Luomai 28’ as tested materials. A complete design of 2 factors and three levels was used. The three N levels were N 120, 180 and 240 kg/hm2 (expressed as N120, N180, N240), and those for Zn (ZnSO4·7H2O) were 0, 20 and 40 kg/hm2 in turn (expressed as Zn0, Zn20, Zn40). At the stages of jointing, booting, filling and maturity, soil pillars of 20 cm × 20 cm × 40 cm were dug up, and the roots inside were washed out for measurement of root dry weight, root length, root surface area, N metabolism related enzyme activities, and Zn and N contents. At maturity, yield and its components were investigated.
In the same N application level, the root dry weight, the root length, the root surface area, and the activities of nitrate reductase, glutamine synthetase, indoleacetic acid oxidase and the concentrations of N and Zn at main growth stages were all highest under Zn application level of 20 kg/hm2, while ribonuclease activity was the lowest. In the same Zn application level, the root length, the root surface area, and the activities of nitrate reductase, glutamine synthetase, indoleacetic acid oxidase and the concentrations of N and Zn at main growth stages were all highest under the N application level of 180 kg/hm2, while ribonuclease activity was the lowest. N and Zn had significant synergistic effect on yield, root dry weight, root length, root surface area, nitrate reductase activity, glutamine synthetase activity, indoleacetic acid oxidase activity, N content and Zn content. In the treatment of middle N and Zn combination, the number of panicle per hectare, grains per panicle and 1000-grain weight of wheat were the highest, and the yield increased by 3.5%–53.4% (2016–2017), 5.3%–54.5% (2017–2018). The grain yield was positively correlated with the root dry weight, the root length, the root surface area, and the activities of nitrate reductase, glutamine synthetase, indoleacetic acid oxidase and the concentrations of N and Zn. And the grain yield was negatively correlated with the activity of ribonuclease.
N and Zn fertilizers significantly affect wheat root dry weight, root length, root surface area, nitrate reductase activity, glutamine synthetase activity, indoleacetate oxidase activity, ribonuclease activity, N and Zn contents. Appropriate levels of N and Zn fertilizer have synergistic effect in promoting the establishment of good root morphology, regulating the physiological activity of roots and the absorption of N and Zn nutrients, thus beneficial to the formation of high yield of wheat.The comprehensive analysis shows that the N180Zn20 treatment is the best combination under the experimental conditions.
| [1] |
Cakmak I. Enrichment of cereal grains with zinc: Agronomic or genetic biofortification?[J]. Plant and Soil, 2008, 302(1-2): 1-17. DOI: 10.1007/s11104-007-9466-3
|
| [2] |
韩金玲, 李雁鸣, 马春英, 王文颇. 施锌对小麦开花后氮、磷、钾、锌积累和运转的影响[J]. 植物营养与肥料学报, 2006, 12(3): 313-320. DOI: 10.3321/j.issn:1008-505X.2006.03.005
Han J L, Li M, Ma C Y, Wang W P. Effect of zinc fertilization on accumulation and transportation of N, P, K and Zn after anthesis of wheat[J]. Journal of Plant Nutrition and Fertilizers, 2006, 12(3): 313-320. DOI: 10.3321/j.issn:1008-505X.2006.03.005
|
| [3] |
冯绪猛, 郭九信, 高丽敏, 等. 氮锌配施对水稻锌的吸收、累积与分配的影响[J]. 南京农业大学学报, 2015, 38(2): 279-287. DOI: 10.7685/j.issn.1000-2030.2015.02.016
Feng X M, Guo J X, Gao L M, et al. Effects of combination use of N and Zn fertilizers on absorption, accumulation and distribution of zinc in rice crop[J]. Journal of Nanjing Agricultural University, 2015, 38(2): 279-287. DOI: 10.7685/j.issn.1000-2030.2015.02.016
|
| [4] |
陆欣春, 田霄鸿, 杨习文, 等. 氮锌配施对不同冬小麦品种产量及锌营养的影响[J]. 中国生态农业学报, 2010, 18(5): 923-928. DOI: 10.3724/SP.J.1011.2010.00923
Lu X C, Tian X H, Yang X W, et al. Effect of combination use of Zn and N fertilizers on yield and Zn content in winter wheat[J]. Chinese Journal of Eco-Agriculture, 2010, 18(5): 923-928. DOI: 10.3724/SP.J.1011.2010.00923
|
| [5] |
康利, 马政华, 李红英, 等. 氮锌配施对玉米干物质积累及产量效应的研究[J]. 中国土壤与肥料, 2011, (1): 34-38. DOI: 10.3969/j.issn.1673-6257.2011.01.008
Kang L Y, Ma Z H, Li H Y, et al. Effect of application of nitrogen and zinc fertilizers on dry material accumulation and yield in maize[J]. Soil and Fertilizer Sciences in China, 2011, (1): 34-38. DOI: 10.3969/j.issn.1673-6257.2011.01.008
|
| [6] |
何忠俊, 洪常青, 梁社往, 等. 氮锌复合作用对黑麦草生长和生理生化特性影响的研究[J]. 土壤通报, 2005, 36(5): 726-730. DOI: 10.3321/j.issn:0564-3945.2005.05.022
He Z J, Hong C Q, Liang S W, et al. Compound effects of N and Zn on physiological-biochemical characteristics of ryegrass[J]. Chinese Journal of Soil Science, 2005, 36(5): 726-730. DOI: 10.3321/j.issn:0564-3945.2005.05.022
|
| [7] |
刘娟花, 国春慧, 陈艳龙, 等. 锌肥种类和施用方式对土壤锌形态及有效性的影响[J]. 西北农林科技大学学报(自然科学版), 2017, 45(4): 149-156.
Liu J H, Yuan C H, Chen Y L, et al. Effect of source and application method on Zn form and availability in soil[J]. Journal of Northwest A&F University (Natural Science Edition), 2017, 45(4): 149-156.
|
| [8] |
薛启, 王康才, 梁永富, 等. 氮锌互作对藿香生长、产量及有效成分的影响[J]. 中国中药杂志, 2018, 43(13): 2654-2663.
Xue Q, Wang K C, Liang Y F, et al. Effects of N and Zn interaction on growth, yield and active components of Agastache rugosa[J]. China Journal of Chinese Materia Medica, 2018, 43(13): 2654-2663.
|
| [9] |
臧贺藏, 王言景, 张均, 等. 冬小麦初生根与次生根形态、生理性状差异分析[J]. 河南农业科学, 2018, 47(6): 18-23.
Zang H Z, Wang Y J, Zhang J, et al. Difference analysis in morphological and physiological characteristics between seminal and nodal roots of winter wheat (Triticum aestivum L.)[J]. Journal of Henan Agricultural Sciences, 2018, 47(6): 18-23.
|
| [10] |
张向前, 曹承富, 陈欢, 等. 长期定位施肥对砂姜黑土小麦根系性状和根冠比的影响[J]. 麦类作物学报, 2017, 37(3): 382-389. DOI: 10.7606/j.issn.1009-1041.2017.03.15
Zhang X Q, Cao C F, Chen H, et al. Effect of long-term fertilization on root traits and root-shoot ratio of wheat in lime concretion black soil[J]. Journal of Triticeae Crops, 2017, 37(3): 382-389. DOI: 10.7606/j.issn.1009-1041.2017.03.15
|
| [11] |
Paezgarcia A, Motes C M, Scheible W, et al. Root traits and phenotyping strategies for plant improvement[J]. Plants, 2015, 4(2): 334-355. DOI: 10.3390/plants4020334
|
| [12] |
Chloupek O, Dostál V, StřEda T, et al. Drought tolerance of barley varieties in relation to their root system size[J]. Plant Breeding, 2010, 129(6): 630-636. DOI: 10.1111/j.1439-0523.2010.01801.x
|
| [13] |
陈晨, 龚海青, 张敬智, 等. 不同基因型水稻苗期氮营养特性差异及综合评价[J]. 中国生态农业学报, 2016, 24(10): 1347-1355.
Chen C, Gong H Q, Zhang J Z, et al. Evaluation of nitrogen nutrition characteristics of different rice cultivars at seedling stage[J]. Chinese Journal of Eco-Agriculture, 2016, 24(10): 1347-1355.
|
| [14] |
张均, 马超, 王贺正, 等. 氮、锌肥对作物根系生长发育调控的研究进展[J]. 湖北农业科学, 2017, 56(5): 801-804.
Zhang J, Ma C, Wang H Z, et al. Study progress of crop root growth and regulation through nitrogen and zinc[J]. Hubei Agricultural Sciences, 2017, 56(5): 801-804.
|
| [15] |
García-Bañuelos M L, Sida-Arreola J P, Sánchez E. Biofortification-promising approach to increasing the content of iron and zinc in staple food crops[J]. Journal of Elementology, 2014, 19(3): 865-888.
|
| [16] |
姜丽娜, 侯飞, 蒿宝珍, 等. Zn2+对小麦幼苗干物质及Zn积累的影响[J]. 麦类作物学报, 2008, 28(6): 1005-1010.
Jiang L N, Hou F, Hao B Z, et al. Effect of Zn2+ on dry matter and zinc accumulation in wheat seedling[J]. Journal of Triticeae Crops, 2008, 28(6): 1005-1010.
|
| [17] |
Zhao P, Yang F, Sui F Q, et al. Effect of nitrogen fertilizers on zinc absorption and translocation in winter wheat[J]. Journal of Plant Nutrition, 2016, 39(9): 1311-1318. DOI: 10.1080/01904167.2015.1106560
|
| [18] |
李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000. 125-127.
Li H S. Principles and techniques of plant physiological and biochemical experiments[M]. Beijing: Higher Education Press, 2000. 125-127.
|
| [19] |
马新明, 李琳, 赵鹏, 等. 土壤水分对强筋小麦‘豫麦34’氮素同化酶活性和籽粒品质的影响[J]. 植物生态学报, 2005, 29(1): 48-53. DOI: 10.3321/j.issn:1005-264X.2005.01.007
Ma X M, Li L, Zhao P, et al. Effect of water control on activities of nitrogen assimilation enzymes and grain quality in winter wheat[J]. Acta Phytoecologica Sinica, 2005, 29(1): 48-53. DOI: 10.3321/j.issn:1005-264X.2005.01.007
|
| [20] |
张志良, 瞿伟菁, 李小方. 植物生理学实验指导(第4版)[M]. 北京: 高等教育出版社, 2009. 100–228.
Zhang Z L, Qu W J, Li X F. Plant physiology experiment guidance (4th Edition)[M]. Beijing: Higher Education Press, 2009. 100–228.
|
| [21] |
龚宏伟. 小麦雄性不育系花粉发育过程中核糖核酸酶活性的变化[J]. 江苏农业科学, 2013, 41(9): 63-65. DOI: 10.3969/j.issn.1002-1302.2013.09.023
Gong H W. Changes of ribonuclease activity during pollen development of male sterile lines in wheat[J]. Jiangsu Agricultural Sciences, 2013, 41(9): 63-65. DOI: 10.3969/j.issn.1002-1302.2013.09.023
|
| [22] |
姜琳琳, 韩立思, 韩晓日, 等. 氮素对玉米幼苗生长、根系形态及氮素吸收利用效率的影响[J]. 植物营养与肥料学报, 2011, 17(1): 247-253. DOI: 10.11674/zwyf.2011.0134
Jiang L L, Han L S, Han X R, et al. Effect of nitrogen on growth, root morphology traits, nitrogen uptake and utilization efficiency of maize seedlings[J]. Journal of Plant Nutrition and Fertilizers, 2011, 17(1): 247-253. DOI: 10.11674/zwyf.2011.0134
|
| [23] |
孙刚, 杨习文, 田霄鸿, 李生秀. 不同基因型冬小麦幼苗对缺锌的敏感性反应[J]. 华北农学报, 2009, 24(6): 54-59. DOI: 10.7668/hbnxb.2009.06.011
Sun G, Yang X W, Tian X H, Li S X. Response of Zinc on wheat seedling of different sensitivity under Zinc deficiency[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(6): 54-59. DOI: 10.7668/hbnxb.2009.06.011
|
| [24] |
王佳, 聂兆君, 扶海超, 等. 外源Zn2+对冬小麦幼苗根系生长及部分氮代谢关键酶的影响[J]. 河南农业大学学报, 2018, 52(3): 307-312.
Wang J, Nie Z J, Fu H C, et al. Effects of exogenous Zn2+ on root growth and some key enzymes in nitrogen metabolism in winter wheat seedlings[J]. Journal of Henan Agricultural University, 2018, 52(3): 307-312.
|
| [25] |
Nie Z J, Zhao P, Wang J, et al. Absorption kinetics and subcellular fractionation of zinc in winter wheat in response to nitrogen supply[J]. Frontiers in Plant Science, 2017, 8: 1-10.
|
| [26] |
Alloway B J. Soil factors associated with zinc deficiency in crops and humans[J]. Environmental Geochemistry and Health, 2009, 31(5): 537-548. DOI: 10.1007/s10653-009-9255-4
|
| [27] |
Améziane R E, Deleens E, Noctor G, et al. Stage of development is an important determinant in the effect of nitrate on photoassimilate (13C) partitioning in chicory (Cichorium intybus)[J]. Journal of Experimental Botany, 1997, 48(306): 25-33.
|
| [28] |
Liu H E, Wang Q Y, Rengel Z, et al. Zinc fertilization alters flour protein composition of winter wheat genotypes varying in gluten content[J]. Plant Soil and Environment, 2015, 61(5): 195-200.
|
| [29] |
Devi S R, Prasad M N V. Ferulic acid mediated changes in oxidative enzymes of maize seedlings: implications in growth[J]. Biologia Plantarum, 1996, 38(3): 387-395 DOI: 10.1007/BF02896668
|
| [30] |
周斌, 张金尧, 乙引, 汪洪. 缺锌对玉米根系发育、生长素含量及生长素转运基因表达的影响[J]. 植物营养与肥料学报, 2017, 23(5): 1352-1358. DOI: 10.11674/zwyf.17062
Zhou B, Zhang J Y, Yi Y, Wang H. Effects of zinc deficiency on root growth, endogenous auxin content and key auxin transport genes expressions in maize roots[J]. Journal of Plant Nutrition and Fertilizers, 23(5): 1352-1358. DOI: 10.11674/zwyf.17062
|
| [31] |
Naik M S, Asana R D. Effect of Zinc deficiency on the synthesis of protein, mineral uptake and ribonuclease activity in the cotton plant[J]. Indian Journal of Plant Physiology, 1961, 4: 103-108.
|
| [32] |
Nag S, Saha K, Choudhuri M A. Role of auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting[J]. Journal of Plant Growth Regulation, 2001, 20(2): 182-194.
|
| [33] |
邱喜阳, 王晨阳, 王彦丽, 等. 施氮量对冬小麦根系生长分布及产量的影响[J]. 西北农业学报, 2012, 21(1): 53-58. DOI: 10.3969/j.issn.1004-1389.2012.01.011
Qiu X Y, Wang C Y, Wang Y L, et al. Effects of nitrogen application rate on root system distribution and grain yield of winter cultivars[J]. Acta Agricultura Boreali-Occidentalis Sinica, 2012, 21(1): 53-58. DOI: 10.3969/j.issn.1004-1389.2012.01.011
|
| [34] |
王树起, 韩晓增, 乔云发, 等. 施氮对大豆根系形态和氮素吸收积累的影响[J]. 中国生态农业学报, 2009, 17(6): 1069-1073.
Wang S Q, Han X Z, Qiao Y F, et al. Root morphology and nitrogen accumulation in soybean (Glycine max L.) under different nitrogen application levels[J]. Chinese Journal of Eco-Agriculture, 2009, 17(6): 1069-1073.
|
| [35] |
Kutman U B, Yildiz B, Ozturk L, et al. Biofortification of durum wheat with zinc through soil and foliar applications of nitrogen[J]. Cereal Chemistry, 2010, 87(1): 1-9. DOI: 10.1094/CCHEM-87-1-0001
|
| [36] |
黄文川, 李录久, 李文高, 等. 小麦氮锌配施效应及增产机理研究[J]. 核农学报, 2000, 14(4): 225-229. DOI: 10.3969/j.issn.1000-8551.2000.04.006
Huang W C, Li L J, Li W G, et al. Effect of applying nitrogen combined with zinc on wheat yield[J]. Journal of Nuclear Agricultural Science, 2000, 14(4): 225-229. DOI: 10.3969/j.issn.1000-8551.2000.04.006
|
| [37] |
Alloway B J. Zinc in soils and crop nutrition (2nd Edition)[M]. Brussels and Paris: IZA and IFA, 2008.
|
| [38] |
聂兆君, 赵鹏, 秦世玉, 等. 氮锌配施对冬小麦根土界面锌有效性及形态分级的影响[J]. 植物营养与肥料学报, 2018, 24(5): 1185-1193. DOI: 10.11674/zwyf.17477
Nie Z J, Zhao P, Qin S Y, et al. Effects of combined application of nitrogen and zinc on the availability and fraction of zinc in root-soil interface of winter wheat[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(5): 1185-1193. DOI: 10.11674/zwyf.17477
|
| [39] |
李秋杰, 贺德先, 周苏玫, 等. 氮锌配施对小麦不同生育期Zn含量变化的影响[J]. 山东农业科学, 2018, 50(11): 76-82.
Li Q J, He D X, Zhou S M, et al. Effect of combined application of nitrogen and zinc on content variation of Zn in wheat at different growth stages[J]. Shandong Agricultural Sciences, 2018, 50(11): 76-82.
|
| [40] |
Aciksoz S B, Yazici A, Ozturk L, et al. Biofortification of wheat with iron through soil and foliar application of nitrogen and iron fertilizers[J]. Plant and Soil, 2011, 349(1-2): 215-225.
|
| [41] |
Xue Y F, Zhang W, Liu D Y, et al. Effects of nitrogen management on root morphology and zinc translocation from root to shoot of winter wheat in the field[J]. Field Crops Research, 2014, 161: 38-45. DOI: 10.1016/j.fcr.2014.01.009
|
| [42] |
刘铮. 我国土壤中锌含量的分布规律[J]. 中国农业科学, 1994, 27(1): 30-37.
Liu Z. The distribution of zinc content in soil in China[J]. Scientia Agricultura Sinica, 1994, 27(1): 30-37.
|
| [43] |
常红, 周鑫斌, 于淑慧, 等. 小麦氮锌配施效应研究[J]. 西南大学学报(自然科学版), 2013, 35(11): 49-53.
Chang H, Zhou X B, Yu S H, et al. Study of the effect of combined application of nitrogen and zinc on wheat[J]. Journal of Southwest University (Natural Science Edition), 2013, 35(11): 49-53.
|
| [44] |
安婷婷, 侯小畔, 周亚男, 等. 氮肥用量对小麦开花后根际土壤特性和产量的影响[J]. 中国农业科学, 2017, 50(17): 3352-3364. DOI: 10.3864/j.issn.0578-1752.2017.17.010
An T T, Hou X P, Zhou Y N, et al. Effects of nitrogen fertilizer rates on rhizosphere soil characteristics and yield after anthesis of wheat[J]. Scientia Agricultura Sinica, 2017, 50(17): 3352-3364. DOI: 10.3864/j.issn.0578-1752.2017.17.010
|
| [45] |
李孟华, 王朝辉, 王建伟, 等. 低锌旱地施锌方式对小麦产量和锌利用的影响[J]. 植物营养与肥料学报, 2013, 19(6): 1346-1355. DOI: 10.11674/zwyf.2013.0608
Li M H, Wang C H, Wang J W, et al. Effect of Zn application methods on wheat grain yield and Zn utilization in Zn-deficient soils of dryland[J]. Journal of Plant Nutrition and Fertilizers, 2013, 19(6): 1346-1355. DOI: 10.11674/zwyf.2013.0608
|
DownLoad: