- ISSN 1008-505X
- CN 11-3996/S
| Citation: |
CHEN Yu, ZHANG Ya-qin, DENG Qiu-lin, YANG Zheng-ming, LEI Fei-yi, FU Yu-ping, LIU Zhen-dong, CHEN Xing-fu. Optimum combining concentration of zinc, boron and molybdenum foliar application for high content of nucleosides in Fritillaria unibracteata var. wabuensis[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(8): 1401-1412. DOI: 10.11674/zwyf.18223
|
Nucleoside component is one of the main active substances of Fritillaria. The contribution rate of Zn, B and Mo application on the content of nucleosides in Fritillaria was studied for the optimization of their application formula of Fritillaria unibracteata var. wabuensis.
A plot experiment was conducted at the standardized production base of Sichuan Fritillaria. The F. unibracteata var. wabuensis bulbs of four-year-old and 12 mm in size were used as materials. A five-level quadratic orthogonal rotation method was used for the treatment design of the experiment. The 20%, 30%, 30% and 20% of the total Zn, B and Mo fertilizers were foliar applied at the budding, early flowering, full flowering and young fruit stages of Fritillaria, respectively. The Fritillaria bulbs were harvested from the experimental plots, and they were used to determine the content of 10 nucleosides.
The least square method, gray correlation degree and DTOPSIS method were used to calculate the contribution rates of trace elements and nucleosides, the influence of the single factors and interaction of two factors on the nucleoside components to obtain the optimal fertilization recommendation.
By contribution rate analysis, zinc fertilizer contributed the most to uridine, thymidine and inosine, boron fertilizer contributed the most to cytidine and guanosine, and molybdenum contributed the most to uracil and thymidine. Single factor analysis showed that zinc fertilizer and boron fertilizer had significant effects on cytidine, and molybdenum fertilizer had significant effect on thymidine. Interaction analysis showed that zinc-boron interaction synergistically exerted uracil, but antagonized thymine, uridine and thymidine; zinc-molybdenum interaction synergized with uracil, uridine and inosine; boron-molybdenum interaction antagonized cytidine, thymidine, guanosine and uridine. The grey correlation degree and DTOPSIS method analysis showed the comprehensive optimal treatment.
The optimal fertilization was determined as zinc fertilizer 8.05–11.85 kg/hm2; boron fertilizer 11.70–16.47 kg/hm2; and molybdenum fertilizer 0.36–0.51 kg/hm2. By using grey correlation and DTOPSIS method, when the zinc fertilizer was 15.71 kg/hm2, boron fertilizer 5.53 kg/hm2 and molybdenum fertilizer 0.65 kg/hm2, the content of 10 nucleosides was optimal.
| [1] |
国家药典委员会. 中华人民共和国药典(2015年版, 一部)[S]. 北京: 中国医药科技社, 2015. 36–37.
National Pharmacopoeia Committee. Chinese pharmacopoeia (2015, Edition I)[S]. Beijing: China Pharmaceutical Technology Corporation, 2015. 36–37.
|
| [2] |
赵高琼, 任波, 董小萍, 等. 川贝母研究现状[J]. 中药与临床, 2012, 3(6): 59–64.
Zhao G Q, Reng B, Dong X P, et al. The Current Status of Fritillaria Fritillariae[J]. Herbal Medicine & Clinical Medicine, 2012, 3(6): 59–64.
|
| [3] |
Hao D C, Gu X J, Xiao P G, et al. Phytochemical and biological research of Fritillaria medicine resources[J]. Chinese Journal of Natural Medicines, 2013, 11(4): 330–344. DOI: 10.1016/S1875-5364(13)60050-3
|
| [4] |
马鹏, 王丽, 孙年喜, 彭锐. HPLC-DAD同时测定太白贝母中9种核苷类成分的含量[J]. 中药材, 2016, 39(1): 124–126.
Ma P, WANG L, SUN N X, Peng Y. Simultaneous determination of 9 nucleoside constituents in Fritillaria taipaiensis by HPLC-DAD[J]. Journal of Chinese Medicinal Materials, 2016, 39(1): 124–126.
|
| [5] |
Pan F, Wu W. Simultaneous determination of 10 nucleosides and nucleobases from different cultivation years of Fritillaria unibracteata var. wabuensis by HPLC-DAD[J]. Journal of Chinese Pharmaceutical Sciences, 2017, 26(5): 346–354.
|
| [6] |
颜晓燕, 童志远, 晏子俊, 等. 暗紫贝母及浙贝母醇水提取物镇咳、祛痰及平喘作用比较研究[J]. 中国实验方剂学杂志, 2012, 18(16): 250-254.
Yang X Y, Tong Z Y, Yan Z J, et al. A comparative study on antitussive, expectorant and anti-asthmatic effects of water extracts of Fritillaria thunbergii and Fritillaria unibracteata[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(16): 250–254.
|
| [7] |
Kang D G, Sohn E J, Lee Y M, et al. Effects of bulbus Fritillaria water extract on blood pressure and renal functions in the L-NAME-induced hypertensive rats[J]. Journal of Ethnopharmacology, 2004, 91(01): 51–56. DOI: 10.1016/j.jep.2003.11.015
|
| [8] |
刘大会, 刘伟, 朱端卫, 等. 施用磷肥对菊花活性成分及清除自由基能力的影响[J]. 中国中药杂志, 2010, 35(17): 2236–2241.
Liu D H, Liu W, Zhu D W, et al. Effect of phosphate fertilizer application on the active ingredients of Chrysanthemum and its ability to scavenge free radicals[J]. China Journal of Chinese Materia Medica, 2010, 35(17): 2236–2241.
|
| [9] |
窦明明, 石峰, 马留辉, 等. 微肥配施对泽泻产量的影响[J]. 中药材, 2017, 40(1): 7–11.
Dou M M, Shi F, Ma L H, et al. Effects of Micro Fertilizers on the Yield of Alismaorientalis[J]. Chinese Medicinal Materials, 2017, 40(1): 7–11.
|
| [10] |
张亚琴, 杨正明, 石峰, 等. 叶面喷施微肥对川白芷主要有效成分含量的影响[J]. 应用生态学报, 2017, 28(11): 3505–3514.
Zhang Y Q, Yang Z M, Shi F, et al. Effect of foliar application of micro-fertilizers on the content of main effective components of Angelica dahurica[J]. The Journal of Applied Ecology, 2017, 28(11): 3505–3514.
|
| [11] |
伍燕华. 川贝母(Fritillaria cirrhosa)栽培中关键技术的初步研究[D]. 成都: 成都中医药大学硕士学位论文, 2013.
Wu Y H. A preliminary study on the key techniques of Fritillaria cirrhosa cultivation[D]. Chengdu: MS Thesis of Chengdu University of Traditional Chinese Medicine, 2013.
|
| [12] |
刘震东, 王曙, 陈心启. 关于瓦布贝母的分类等级研究[J]. 植物分类与资源学报, 2009, 31(2): 145–145.
Liu Z D, Wang S, Chen X Q. Research on the classification of Fritillaria unibracteata var. wabuensis[J]. Journal of Plant Classification and Resources, 2009, 31(2): 145–145.
|
| [13] |
刘辉, 陈士林, 姚辉, 等. 川贝母的资源学研究进展[J]. 中国中药杂志, 2008, 33(14): 1645–1648.
Liu H, Chen S L, Yao H, et al. The development of resource research of Fritillaria cirrhosa[J]. Chinese Journal of Chinese Materia Medica, 2008, 33(14): 1645–1648.
|
| [14] |
杨晓云, 陈良. 叶片吸肥特性及叶面肥施用技术[J]. 吉林农业, 2011, (8): 92–92. DOI: 10.3969/j.issn.1674-0432.2011.08.089
Yang X Y, Chen L. Leaves sucking fertilizer characteristics and foliar fertilizer application technology[J]. Jilin Agricultural Sciences, 2011, (8): 92–92. DOI: 10.3969/j.issn.1674-0432.2011.08.089
|
| [15] |
张耀鸿. 微量元素肥料的功能以及施用方法[J]. 安徽农学通报, 2007, 13(19): 120–121. DOI: 10.3969/j.issn.1007-7731.2007.19.058
Zheng Y H. The Functions of trace element fertilizers and application methods[J]. Anhui Agricultural Science Bulletin, 2007, 13(19): 120–121. DOI: 10.3969/j.issn.1007-7731.2007.19.058
|
| [16] |
蒋向辉, 佘朝文, 苑静. 微量元素对金银花绿原酸合成关键酶基因LjHCT和LjC3H1表达的影响研究[J]. 植物科学学报, 2017, 35(2): 260–266.
Jiang X H, She C W, Yuan J. Effects of trace elements on the expression of LjHCT and LjC3H1, key enzyme genes for synthesis of chlorogenic acid in honeysuckle[J]. Journal of Plant Science, 2017, 35(2): 260–266.
|
| [17] |
孟杰, 陈兴福, 杨文钰, 等. 锌硼钼配施对北柴胡产量及柴胡皂苷a, d含量的影响[J]. 中国中药杂志, 2014, 39(22): 4297–4303.
Meng J, Chen X F, Yang W Y, et al. The effect of boron and molybdenum combined application on the yield of Bupleurumchinense D C. and the content of saikosaponin a and d[J]. China Journal of Chinese Materia Medica, 2014, 39(22): 4297–4303.
|
| [18] |
刘美英, 高炳德, 吕福虎. 锌肥、硼肥及其交互作用对春小麦籽粒品质的影响[J]. 干旱区资源与环境, 2016, 30(1): 175–179.
Liu M Y, Gao B D, LYU F F. Effects of zinc fertilizer, boron fertilizer and their interaction on grain quality of spring wheat[J]. Arid Land Resources and Environment, 2016, 30(1): 175–179.
|
| [19] |
张志伟. 锌、钼对水分逆境下夏玉米产量形成及品质的影响[D].郑州: 河南农业大学硕士学位论文, 2013.
Zhang Z W. Effect of zinc and molybdenum on yield formation and quality of summer maize under water stress[D]. zhengzhou: MS Thesis of Henan Agricultural University, 2013.
|
| [20] |
蒋春姬, 王宁, 王晓光, 等. 钙钼硼肥对花生生长发育及产量品质的影响[J]. 中国油料作物学报, 2017, 39(4): 524-531. DOI: 10.7505/j.issn.1007-9084.2017.04.014
Jiang C J, Wang N, Wang X G, et al. Effects of calcium, molybdenum and boron fertilizers on peanut growth, yield and quality[J]. Chinese Journal of Oil Crops, 2017, 39(4): 524–531. DOI: 10.7505/j.issn.1007-9084.2017.04.014
|
| [21] |
张元珍. 供锌水平对苹果树叶片光合特性、碳水化合物及其代谢酶活性的影响[D]. 泰安: 山东农业大学硕士学位论文, 2011.
Zhang Y Z. Effects of zinc supply level on photosynthetic characteristics, carbohydrate and metabolic enzyme activities of apple tree leaves[D]. Tai'an: MS Thesis of Shandong Agricultural University, 2011.
|
| [22] |
杨系玲, 杨克军, 李佐同, 等. 锌对不同基因型玉米幼苗光合特性及锌积累的影响[J]. 核农学报, 2016, 30(3): 571–579. DOI: 10.11869/j.issn.100-8551.2016.03.0571
Yang X L, Yang K J, Li Z T, et al. Effects of zinc on photosynthetic characteristics and zinc accumulation in different genotype maize seedlings[J]. Chinese Journal of Nuclear Agriculture, 2016, 30(3): 571–579. DOI: 10.11869/j.issn.100-8551.2016.03.0571
|
| [23] |
翁伯琦, 黄东风, 王义祥, 等. 施硼对圆叶决明植株生长、养分含量及固氮能力的影响[J]. 热带亚热带植物学报, 2007, 15(4): 343–348. DOI: 10.3969/j.issn.1005-3395.2007.04.011
Wong B Q, Huang D F, Wang Y X, et al. Effect of boron on growth, nutrient content and nitrogen-fixing ability of C. obtusifolia Plants[J]. Journal of Tropical and Subtropical Botany, 2007, 15(4): 343–348. DOI: 10.3969/j.issn.1005-3395.2007.04.011
|
| [24] |
武松伟, 胡承孝, 谭启玲, 等. 施钼对冬小麦钼高、低效品种氮代谢的影响[J]. 华中农业大学学报, 2015, 34(5): 52–57.
Wu S W, Hu C X, Tan Q L, et al. Effect of molybdenum on nitrogen metabolism in molybdenum and inefficient wheat varieties[J]. Journal of Huazhong Agricultural University, 2015, 34(5): 52–57
|
| [25] |
张翔, 毛家伟, 司贤宗, 李国平. 不同种类有机肥与钼肥配施对连作花生生长发育及产量、品质的影响[J]. 中国油料作物学报, 2014, 36(4): 489–493. DOI: 10.7505/j.issn.1007-9084.2014.04.009
Zhang X, Mao J W, Si X Z, Li G P. Effect of different kinds of organic fertilizer and molybdenum fertilizer on growth, yield and quality of continuous cropping peanut[J]. Chinese Journal of Oil Crops, 2014, 36(4): 489–493. DOI: 10.7505/j.issn.1007-9084.2014.04.009
|
| [26] |
游静, 张德全, 潘兴娇, 等. 高效液相色谱法同时测定太白贝母与暗紫贝母中9种核苷类成分的含量[J]. 食品与发酵工业, 2016, 42(1): 174–179.
You J, Zhang D Q, Pan X J, et al. Simultaneous determination of 9 nucleoside concentrations in Fritillaria taipaiensis and Fritillaria unibracteata by high performance liquid chromatography[J]. Food and Fermentation Industries, 2016, 42(1): 174–179.
|
| [1] | SU Xin-yue, WANG Jin-feng, SUN Nan, KUAI Yan, FAN Zhi-yong, ZHANG Jiu-quan, XU Ming-gang. Temporal and spatial variation of soil available zinc, molybdenum and boron contents in Dali tobacco-growing fields and the influencing factors[J]. Journal of Plant Nutrition and Fertilizers, 2023, 29(12): 2371-2380. DOI: 10.11674/zwyf.2023224 |
| [2] | ZHANG Ya-qin, LEI Fei-yi, CHEN Yu, LI Si-jia, DOU Ming-ming, MA Liu-hui, SHI Feng, CHEN Xing-fu. Effect of combined fertilization of zinc, boron and molybdenum on agronomic traits and yield of Angelica dahurica[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(3): 769-778. DOI: 10.11674/zwyf.17445 |
| [3] | SUN Guo-feng, SHENG Jing, ZHANG Li-ping, ZHOU Wei, CHEN Liu-gen, ZHENG Jian-chu. Safety dosage of pig manure based on zinc accumulation in rice and wheat rotated farmland[J]. Journal of Plant Nutrition and Fertilizers, 2017, 23(1): 174-180. DOI: 10.11674/zwyf.16257 |
| [4] | YAO Zhi-yuan, WANG Zheng, LI Jing, YU Chang-wei, CAO Qun-hu, CAO Wei-dong, GAO Ya-jun. Profit analysis of different rotations based on leguminous green manure in dryland area[J]. Journal of Plant Nutrition and Fertilizers, 2016, 22(1): 76-84. DOI: 10.11674/zwyf.14432 |
| [5] | LIANG Xin-hua, ZHANG Feng-xia, WANG Jun, HE Wen-shou. Effects of molybdenum，boron，manganese and zinc on quality of cultured Glycyrrhiza uralensis Fisch[J]. Journal of Plant Nutrition and Fertilizers, 2011, 17(6): 1487-1494. DOI: 10.11674/zwyf.2011.1042 |
| [6] | MA Xin, SHI Tao-xiong, WU Ji, LU Jian-wei, SHI Lei, XU Fang-sen. Effect of different boron fertilizers on seed yield and seed quality of oilseed rape (Brassica napus) and their residual effectiveness for rotated rice[J]. Journal of Plant Nutrition and Fertilizers, 2011, 17(3): 761-766. DOI: 10.11674/zwyf.2011.0322 |
| [7] | ZHU Qi-hong, XIA Hong-xia. A quadratic regression model on the removal of fluorine from tea soil[J]. Journal of Plant Nutrition and Fertilizers, 2009, 15(5): 1122-1129. DOI: 10.11674/zwyf.2009.0519 |
| [8] | LIU Peng, YANG Yu-ai. Effect of molybdenum and boron on photosynthetic efficiency of soybean[J]. Journal of Plant Nutrition and Fertilizers, 2003, 9(4): 456-461. DOI: 10.11674/zwyf.2003.0415 |
| [9] | LIU Peng, YANG Yu. EFFECT OF MOLYBDENUM AND BORON ON NITROGEN METABOLISM OF SOYBEA[J]. Journal of Plant Nutrition and Fertilizers, 1999, 5(4): 347-351. DOI: 10.11674/zwyf.1999.0409 |
| [10] | Shen Jianbo Li Rengang, . USING ORTHOGONAL TREND ANALYSIS TO DEVELOP THE LARGE AREA ECONOMIC FERTILIZATION MODELS[J]. Journal of Plant Nutrition and Fertilizers, 1999, 5(3): 258-262. DOI: 10.11674/zwyf.1999.0309 |
DownLoad: