- ISSN 1008-505X
- CN 11-3996/S
| Citation: |
LI Xiu-li, ZHENG Xu-feng, JIANG Yuan-mao, WEI Shao-chong. Effect of ethephon on absorption, distribution and utilization of nitrate-nitrogen by T337 apple dwarf rootstock seedlings[J]. Journal of Plant Nutrition and Fertilizers, 2019, 25(7): 1204-1210. DOI: 10.11674/zwyf.18267
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The effect of foliar spray of ethephon on the absorption, utilization and distribution of 15N-NO3− by seedlings of T337 apple dwarf rootstock was studied, to provide a reference for its rational application in apple production.
The seedlings of T337 were used as the tested materials in a pot experiment, in which the nutrients were supplied with Hoagland nutrient solution containing 10 mmol/L of NO3−. The seedlings were cultured in normal solution until 10 leaves, and stopped nutrient supply for 7 days for ethephon treatment. The ethephon treatment concentrations were 200 μL/L (E1) and 400 μL/L (E2), using water spray as control (CK). Seedling samples were taken at the 0, 4, 8, 12, 16 days for the measurement of the root activity and the activities of nitrate reductase (NR) and glutamine synthetase (GS) in roots and leaves. At the end of experiment (20 days after treatment), the absorption, distribution and utilization of 15N by seedlings were determined.
The root activity of each treatment increased first and then decreased with the prolongation of the experiment time, and reached the maximum at the 8th day. The root activities in E1 and E2 were significantly higher than that in the control, and that of E2 was significantly higher than that of E1 as well. The activities of NR and GS in both the roots and leaves of all the three treatments increased first and then decreased within the treatment period, with the peak at the 8th day. The activities of the two enzymes in the roots of seedlings in E1 and E2 treatments were higher than those in CK, but those in the leaves were higher only within the first 12 days, and became lower afterward. The NR activity of leaf in E1 and CK were higher than that of root during the monitoring period, while the phenomenon only appeared in the first 12 days in E2, and became opposite from 16 days to 20 days. Ethephon treatment significantly increased the biomass of seedlings. The dry leaf biomass of E1 was significantly higher than that of CK. The root and leaf dry biomass of E2 were 87.0% and 28.8% higher than those of CK, and the root-shoot ratio of seedlings was significantly increased as result. The 15N utilization rates in E1 and E2 treatments were increased by 3.44 and 15.32 percentage points, and the root distribution rates were increased by 33.3% and 67.4% respectively, compared with CK.
Foliar spray of ethephon could affect the activity of nitrate reductase and glutamine synthetase in root and leaf of T337 seedling, increase the biomass and root-shoot ratio, and lead to significant increase of the use of nitrate nitrogen and increase of the distribution rate of 15N in seedling root. Among them, the best result was treated with 400 μL/L ethephon.
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