CHEN Lei, ZHU Yue-lin, YANG Li-fei, WANG Cong. Effects of nitrogen forms and ratios on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean[J]. Journal of Plant Nutrition and Fertilizers, 2010, 16(3): 768-772. DOI: 10.11674/zwyf.2010.0337
Citation:
CHEN Lei, ZHU Yue-lin, YANG Li-fei, WANG Cong. Effects of nitrogen forms and ratios on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean[J]. Journal of Plant Nutrition and Fertilizers, 2010, 16(3): 768-772. DOI: 10.11674/zwyf.2010.0337
CHEN Lei, ZHU Yue-lin, YANG Li-fei, WANG Cong. Effects of nitrogen forms and ratios on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean[J]. Journal of Plant Nutrition and Fertilizers, 2010, 16(3): 768-772. DOI: 10.11674/zwyf.2010.0337
Citation:
CHEN Lei, ZHU Yue-lin, YANG Li-fei, WANG Cong. Effects of nitrogen forms and ratios on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean[J]. Journal of Plant Nutrition and Fertilizers, 2010, 16(3): 768-772. DOI: 10.11674/zwyf.2010.0337
Using the vermiculite culture, the effects of ratios of NO-3-N and NH+4-N on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean [Glycine max (L.) Merr. cv. Li-xiang 95-1] were studied. The results show that the appropriate ratio of NO-3-N and NH+4-N is about 75∶25 which is beneficial to the growth and development of the soybean, and produces the maximum plant biomass. Under the treatment of excessive NO-3 (100%) or NH+4 (75%), both biomass production and yields are decreased obviously, especially for the NH+4 (75%) treatment. In the NO-3∶NH+4 treatments of 75∶25 and 50∶50, the activities of antioxidant enzymes are low, and the O2 producing rate, hydrogen peroxide (H2O2) and malondiadehyde (MDA) contents are also low, therefore the degree of oxidative stress is comparatively low. However, under the NO-3∶NH+4 treatment of 25∶75, the antioxidant enzyme activities, the O2 producing rate, H2O2 and MDA contents reach to their highest values. These results indicate that excessive NH+4 is harmful to cell membrane integrity, resulting in severe degree of oxidative damage in the seeds of vegetable soybean.