- ISSN 1008-505X
- CN 11-3996/S
| Citation: |
YAN Guo-chao, FAN Xiao-ping, TAN Li, YIN Chang, LIANG Yong-chao. Exogenous silicon effectively enhances salt stress resistance of rice by upregulating antioxidant enzymes activities and expression of genes related to Na/K homeostasis[J]. Journal of Plant Nutrition and Fertilizers, 2020, 26(11): 1935-1943. DOI: 10.11674/zwyf.20154
|
Salt stress is one of the global abiotic obstacles severely limiting crop growth. Exogenous silicon (Si) has been proven to effectively enhance salt stress resistance in many plant species. To investigate the mechanisms of Si-induced salt stress resistance, we assessed the effects of Si on the activities of antioxidant enzymes and the expression of Na/K homeostasis related genes in rice.
Rice (Oryza sativa L. cv. Nipponbare) was grown hydroponically with both NaCl (0, 50 and 100 mmol/L) and Na2SiO3 levels (0, 0.5, 1.5 mmol/L). After five days of treatment, plant length and biomass, photosynthesis and transpiration rates, chlorophyll and malondialdehyde concentrations, and Na/K concentrations were measured. Then, the activities of antioxidant enzymes and the expression of Na/K homeostasis related genes in rice grown with 0 and 100 mmol/L NaCl with or without the addition of 1.5 mmol/L Si were measured.
Salt stress significantly inhibited the growth of both shoot and root, while Si addition enhanced the length and dry weight of shoot but not those of root in rice under salt stress. In addition, exogenous Si addition alleviated salt stress-induced decline in photosynthesis rate and chlorophyll concentration and accumulation of MDA, with no significant impact on transpiration rate. As for Na/K homeostasis, salt stress caused an increment in Na concentration and a decrement in K concentration in both shoot and root. Si decreased Na concentration in shoot but not in root. The concentration of K was not affected by Si in both shoot and root. In general, treatment with salt at a higher level (100 mmol/L NaCl) affected rice growth, photosynthesis system, membrane stabilization and Na/K homeostasis more negatively than at a lower level (50 mmol/L NaCl), while salt stress was more effectively alleviated by Si added at a higher level of Si (1.5 mmol/L Si) than at a lower level (0.5 mmol/L Si). Under 100 mmol/L NaCl salt stress, addition of 1.5 mmol/L Si improved SOD, CAT and APX activity but not POD activity. The expression of K uptake genes (OsHAK1, OsHAK7, OsHAK11 and OsHAK12), Na exclusion gene (OsSOS1) and Na compartmentation genes (OsNHX1, OsNHX3 and OsNHX5) was improved by the addition of 1.5 mmol/L Si under 100 mmol/L NaCl.
Si can regulate antioxidant enzymes activities and Na/K homeostasis related genes expression, thereby alleviating salt stress in rice. 1.5 mmol/L of Si is more effective than 0.5 mmol/L in alleviating salt stress in rice.
| [1] |
Munns R, Tester M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008, 59: 651-681. DOI: 10.1146/annurev.arplant.59.032607.092911
|
| [2] |
Zhu Y X, Gong H J. Beneficial effects of silicon on salt and drought tolerance in plants[J]. Agronomy for Sustainable Development, 2014, 34(2): 455-472. DOI: 10.1007/s13593-013-0194-1
|
| [3] |
Munns R, Gilliham M. Salinity tolerance of crops – what is the cost?[J]. New Phytologist, 2015, 208(3): 668-673. DOI: 10.1111/nph.13519
|
| [4] |
Liang Y C, Sun W C, Zhu Y G, et al. Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: A review[J]. Environmental Pollution, 2007, 147(2): 422-428. DOI: 10.1016/j.envpol.2006.06.008
|
| [5] |
Shi Y, Wang Y C, Flowers T J, et al. Silicon decreases chloride transport in rice (Oryza sativa L.) in saline conditions[J]. Journal of Plant Physiology, 2013, 170(9): 847-853. DOI: 10.1016/j.jplph.2013.01.018
|
| [6] |
Gong H J, Randall D P, Flowers T J. Silicon deposition in the root reduces sodium uptake in rice (Oryza sativa L.) seedlings by reducing bypass flow[J]. Plant, Cell & Environment, 2006, 29(10): 1970-1979.
|
| [7] |
Bosnic P, Bosnic D, Jasnic J, et al. Silicon mediates sodium transport and partitioning in maize under moderate salt stress[J]. Environmental and Experimental Botany, 2018, 155: 681-687. DOI: 10.1016/j.envexpbot.2018.08.018
|
| [8] |
Liang Y C. Effects of silicon on enzyme activity and sodium, potassium and calcium concentration in barley under salt stress[J]. Plant and Soil, 1999, 209(2): 217-224. DOI: 10.1023/A:1004526604913
|
| [9] |
Liang Y C, Zhang W H, Chen Q, et al. Effects of silicon on H+-ATPase and H+-PPase activity, fatty acid composition and fluidity of tonoplast vesicles from roots of salt-stressed barley (Hordeum vulgare L.)[J]. Environmental and Experimental Botany, 2005, 53(1): 29-37. DOI: 10.1016/j.envexpbot.2004.02.010
|
| [10] |
Tuna A L, Kaya C, Higgs D, et al. Silicon improves salinity tolerance in wheat plants[J]. Environmental and Experimental Botany, 2008, 62(1): 10-16. DOI: 10.1016/j.envexpbot.2007.06.006
|
| [11] |
Saqib M, Zorb C, Schubert S. Silicon-mediated improvement in the salt resistance of wheat (Triticum aestivum) results from increased sodium exclusion and resistance to oxidative stress[J]. Functional Plant Biology, 2008, 35(7): 633-639. DOI: 10.1071/FP08100
|
| [12] |
Liang Y C, Hua H X, Zhu Y G, et al. Importance of plant species and external silicon concentration to active silicon uptake and transport[J]. New Phytologist, 2006, 172(1): 63-72. DOI: 10.1111/j.1469-8137.2006.01797.x
|
| [13] |
Liu P, Yin L N, Deng X P, et al. Aquaporin-mediated increase in root hydraulic conductance is involved in silicon-induced improved root water uptake under osmotic stress in Sorghum bicolor L.[J]. Journal of Experimental Botany, 2014, 65(17): 4747-4756. DOI: 10.1093/jxb/eru220
|
| [14] |
Ma J F, Tamai K, Yamaji N, et al. A silicon transporter in rice[J]. Nature, 2006, 440(7084): 688-691. DOI: 10.1038/nature04590
|
| [15] |
Wang H S, Yu C, Fan P P, et al. Identification of two cucumber putative silicon transporter genes in Cucumis sativus[J]. Journal of Plant Growth Regulation, 2015, 34(2): 332-338. DOI: 10.1007/s00344-014-9466-5
|
| [16] |
Wu J W, Guo J, Hu Y H, et al. Distinct physiological responses of tomato and cucumber plants in silicon-mediated alleviation of cadmium stress[J]. Frontiers in Plant Science, 2015, 6: 453.
|
| [17] |
Wu X Y, Yu Y G, Baerson S R, et al. Interactions between nitrogen and dilicon in rice and their effects on resistance toward the brown planthopper Nilaparvata lugens[J]. Frontiers in Plant Science, 2017, 8: 28.
|
| [18] |
Porra R J, Thompson W A, Kriedemann P E. Determination of accurate extinction coefficients and simultaneous-equations for assaying chlorophyll-a and chlorophyll-b extracted with 4 different solvents-verification of the concentration of chlorophyll standards by atomic-absorption spectroscopy[J]. Biochimica Et Biophysica Acta, 1989, 975(3): 384-394. DOI: 10.1016/S0005-2728(89)80347-0
|
| [19] |
Heath R L, Packer L. Photoperoxidation in isolated chloroplasts. I. kinetics and stoichiometry of fatty acid peroxidation[J]. Archives of Biochemistry and Biophysics, 1968, 125(1): 189-198. DOI: 10.1016/0003-9861(68)90654-1
|
| [20] |
Giannopolitis C N, Ries S K. Superoxide dismutases. 1. Occurrence in higher plants[J]. Plant Physiology, 1977, 59(2): 309-314. DOI: 10.1104/pp.59.2.309
|
| [21] |
Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves[J]. Plant Physiology, 1992, 98(4): 1222-1227. DOI: 10.1104/pp.98.4.1222
|
| [22] |
Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J]. Plant & Cell Physiology, 1981, 22(5): 867-880.
|
| [23] |
Liang Y C, Chen Q, Liu Q, et al. Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.)[J]. Journal of Plant Physiology, 2003, 160(10): 1157-1164. DOI: 10.1078/0176-1617-01065
|
| [24] |
Okada T, Nakayama H, Shinmyo A, et al. Expression of OsHAK genes encoding potassium ion transporters in rice[J]. Plant Biotechnology, 2008, 25(3): 241-245. DOI: 10.5511/plantbiotechnology.25.241
|
| [25] |
Porcel R, Aroca R, Azcon R, et al. Regulation of cation transporter genes by the arbuscular mycorrhizal symbiosis in rice plants subjected to salinity suggests improved salt tolerance due to reduced Na(+) root-to-shoot distribution[J]. Mycorrhiza, 2016, 26(7): 673-684. DOI: 10.1007/s00572-016-0704-5
|
| [26] |
Yang T, Zhang S, Hu Y, et al. The role of a potassium transporter OsHAK5 in potassium acquisition and transport from roots to shoots in rice at low potassium supply levels[J]. Plant Physiology, 2014, 166(2): 945-959. DOI: 10.1104/pp.114.246520
|
| [27] |
Yan G C, Nikolic M, Ye M J, et al. Silicon acquisition and accumulation in plant and its significance for agriculture[J]. Journal of Integrative Agriculture, 2018, 17(10): 2138-2150. DOI: 10.1016/S2095-3119(18)62037-4
|
| [28] |
Byrt C S, Munns R. Living with salinity[J]. New Phytologist, 2008, 179(4): 903-905. DOI: 10.1111/j.1469-8137.2008.02596.x
|
| [29] |
Munns R. Comparative physiology of salt and water stress[J]. Plant, Cell & Environment, 2002, 25(2): 239-250.
|
| [30] |
Ma J F. Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses[J]. Soil Science and Plant Nutrition, 2004, 50(1): 11-18. DOI: 10.1080/00380768.2004.10408447
|
| [31] |
Zhu Y X, Gong H J, Yin J L. Role of silicon in mediating salt tolerance in plants: a review[J]. Plants, 2019, 8: 147. DOI: 10.3390/plants8060147
|
| [32] |
Yan G, Fan X, Peng M, et al. Silicon improves rice salinity resistance by alleviating ionic toxicity and osmotic constraint in an organ-specific pattern[J]. Frontiers in Plant Science, 2020, 11: 260. DOI: 10.3389/fpls.2020.00260
|
| [33] |
Zhu Z J, Wei G Q, Li J, et al. Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.)[J]. Plant Science, 2004, 167(3): 527-533. DOI: 10.1016/j.plantsci.2004.04.020
|
| [34] |
Zhu J K. Regulation of ion homeostasis under salt stress[J]. Current Opinion in Plant Biology, 2003, 6(5): 441-445. DOI: 10.1016/S1369-5266(03)00085-2
|
| [35] |
Zhu J K. Plant salt tolerance[J]. Trends in Plant Science, 2001, 6(2): 66-71. DOI: 10.1016/S1360-1385(00)01838-0
|
| [36] |
Kronzucker H J, Britto D T. Sodium transport in plants: a critical review[J]. New Phytologist, 2011, 189(1): 54-81. DOI: 10.1111/j.1469-8137.2010.03540.x
|
| [37] |
Chen G, Hu Q D, Luo L, et al. Rice potassium transporter OsHAK1 is essential for maintaining potassium-mediated growth and functions in salt tolerance over low and high potassium concentration ranges[J]. Plant, Cell & Environment, 2015, 38(12): 2747-2765.
|
| [38] |
Banuelos M A, Garciadeblas B, Cubero B, et al. Inventory and functional characterization of the HAK potassium transporters of rice[J]. Plant Physiology, 2002, 130(2): 784-795. DOI: 10.1104/pp.007781
|
| [39] |
Obata T, Kitamoto H K, Nakamura A, et al. Rice Shaker potassium channel OsKAT1 confers tolerance to salinity stress on yeast and rice cells[J]. Plant Physiology, 2007, 144(4): 1978-1985. DOI: 10.1104/pp.107.101154
|
| [40] |
Shen Y, Shen L, Shen Z, et al. The potassium transporter OsHAK21 functions in the maintenance of ion homeostasis and tolerance to salt stress in rice[J]. Plant Cell and Environment, 2015, 38(12): 2766-2779. DOI: 10.1111/pce.12586
|
| 1. |
杨岩，刘丽，石璟，江丽华，李妮，徐钰. 基于商品价值和环境风险的西葫芦氮肥投入阈值研究. 植物营养与肥料学报. 2025(01): 167-175 .
 本站查看
| |
| 2. |
李蕤颀，李青芮. 利用资源经济推动农业产业发展的路径探索——以云南维西傈僳族自治县中药材产业为例. 云南农业大学学报(社会科学). 2025(02): 157-162 .
| |
| 3. |
阿拉腾其木格. 干旱区农业面源污染防控措施探究——基于巴彦淖尔市X镇的调查. 山西农经. 2024(18): 152-154+179 .
| |
| 4. |
王蕊，李董，张慧，许宁，张敏，温雯. 乡村振兴背景下我国农业废弃物资源化利用现状与发展方向. 农业科技管理. 2024(06): 1-4 .
| |
| 5. |
杨秉庚，刘梦娇，丁文成，徐新朋，李明悦，何萍，周卫. 基于产量反应和农学效率的番茄智能化推荐施肥方法研究. 植物营养与肥料学报. 2024(12): 2296-2306 .
本站查看
|
DownLoad: