• ISSN 1008-505X
  • CN 11-3996/S
黄伟祥, 代雯慈, 陈甜甜, 蔡洪梅, 翁颖, 唐志伟, 尹春, 王捧娜, 郑宝强, 李金才, 陈翔. 喷施KH2PO4提高倒春寒胁迫小麦功能叶片和根系的生理活性[J]. 植物营养与肥料学报, 2024, 30(6): 1222-1233. DOI: 10.11674/zwyf.2024030
引用本文: 黄伟祥, 代雯慈, 陈甜甜, 蔡洪梅, 翁颖, 唐志伟, 尹春, 王捧娜, 郑宝强, 李金才, 陈翔. 喷施KH2PO4提高倒春寒胁迫小麦功能叶片和根系的生理活性[J]. 植物营养与肥料学报, 2024, 30(6): 1222-1233. DOI: 10.11674/zwyf.2024030
HUANG Wei-xiang, DAI Wen-ci, CHEN Tian-tian, CAI Hong-mei, WENG Ying, TANG Zhi-wei, YIN Chun, WANG Peng-na, ZHENG Bao-qiang, LI Jin-cai, CHEN Xiang. Spraying KH2PO4 enhances the physiological activity of wheat flag leaves and roots to alleviate the damage caused by late spring coldness[J]. Journal of Plant Nutrition and Fertilizers, 2024, 30(6): 1222-1233. DOI: 10.11674/zwyf.2024030
Citation: HUANG Wei-xiang, DAI Wen-ci, CHEN Tian-tian, CAI Hong-mei, WENG Ying, TANG Zhi-wei, YIN Chun, WANG Peng-na, ZHENG Bao-qiang, LI Jin-cai, CHEN Xiang. Spraying KH2PO4 enhances the physiological activity of wheat flag leaves and roots to alleviate the damage caused by late spring coldness[J]. Journal of Plant Nutrition and Fertilizers, 2024, 30(6): 1222-1233. DOI: 10.11674/zwyf.2024030

喷施KH2PO4提高倒春寒胁迫小麦功能叶片和根系的生理活性

Spraying KH2PO4 enhances the physiological activity of wheat flag leaves and roots to alleviate the damage caused by late spring coldness

  • 摘要:
    目的 倒春寒是影响黄淮麦区小麦产量形成的主要灾害,喷施磷酸二氢钾(KH2PO4)被广泛应用于减轻倒春寒对小麦的伤害。研究喷施磷酸二氢钾对低温胁迫下小麦花后功能叶与根系生理活性的调控效应,为小麦生产中的防灾减灾提供理论支撑。
    方法 盆栽试验在田间条件下进行,选用抗倒春寒性强的小麦品种烟农19 (YN)和抗倒春寒性弱的小麦品种新麦26 (XM)为试验材料,设置常温对照+喷施清水(CK)、倒春寒处理+喷施清水(T1)、倒春寒处理+喷施KH2PO4 (T2) 3个处理。在小麦幼穗分化到四分体期,将T1 和T2处理的盆栽移入人工气候室内(−4℃)处理4 h,然后移出,24 h后进行喷施处理。于小麦花后7、14、21、28 天测定旗叶SPAD值、根系活力、旗叶与根系的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量。通过主成分分析(PCA),综合评价倒春寒对小麦花后28天地上部与地下部生理活性指标的影响。
    结果 与CK处理相比,两小麦品种T1处理的旗叶SPAD值和抗氧化酶活性分别显著降低了13.94%~35.03%、16.09%~32.84% (SOD)、18.16%~50.10% (POD)和19.21%~75.40% (CAT),MDA含量显著增加了29.55%~124.56%;T1处理的根系活力显著降低了44.00%~75.63%,SOD和POD活性分别显著降低了42.42%~86.11%和31.00%~66.67%,MDA含量增加了61.54%~330.11%。与T1相比,两品种T2处理的旗叶SPAD值显著增加了7.90%~23.38%,SOD、POD和CAT活性分别显著增加了10.63%~32.28%、14.20%~57.20%和10.75%~115.56%,MDA含量显著降低了6.66%~36.08%;根系活力上升了44.71%~139.75%,根系SOD、POD活性分别显著增加了12.13%~102.01%和21.05%~146.61%,根系MDA含量显著降低了12.01%~51.23%。选定的花后28 天地上部与地下部9个生理活性指标之间关系密切,一个主成分其可解释9个变量82.82%的变化,其综合生理活性指数的排序为:YNCK>XMCK>YNT2>XMT2>YNT1>XMT1。
    结论 受倒春寒影响后,施用KH2PO4可提高小麦花后旗叶SPAD值、根系活力和抗氧化酶活性,降低旗叶和根系的MDA含量,从而延缓旗叶和根系的衰老,减轻倒春寒对小麦花后旗叶和根系生理活性的不利影响。

     

    Abstract:
    Objectives In North China Plain, winter wheat often suffers late spring coldness during the jointing stage, foliar application of potassium dihydrogen phosphate (KH2PO4) is a widely used technology to improve the resistance. We investigated the regulatory effect of KH2PO4 on the physiological activities of functional leaves and roots of wheat after anthesis to provide theoretical support for disaster prevention and mitigation in wheat production.
    Methods A pot experiment was conducted under field condition, the wheat cultivar Yanong 19 (YN) with strong cold resistance and Xinmai 26 (XM) with weak cold resistance were selected as the test materials. The experiment was composed of spraying water without late spring coldness treatment (CK), spraying water with late spring coldness treatments (T1) and spraying KH2PO4 with late spring coldness treatment (T2). During the panicle differentiation stage to metaphase, the pots in T1 and T2 treatments were moved into artificial climate chamber and subjected to −4°C for 4 hours, then moved out. On the following day of cold treatment, water or KH2PO4 were sprayed as designed. At 7, 14, 21, and 28 days after anthesis, the SPAD values of flag leaves, root activity, and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde content (MDA) in flag leaves and roots were measured. Principal component analysis (PCA) was employed to construct a comprehensive evaluation model for aboveground and root physiological activities in 28 days after anthesis under late spring coldness.
    Results Compared to CK in flag leaves, T1 treatment significantly reduced SPAD by 13.94%−35.03%, decreased the activity of SOD, POD, and CAT by 16.09%−32.84%, 18.16%−50.10%, and 19.21%−75.40%, respectively, and increased MDA content by 29.55%−124.56%. And in roots, T1 treatment significantly reduced root activity by 44.00%−75.63%, decreased the activities of SOD and POD by 42.42%−86.11%, and 31.00%−66.67%, but increased MDA content by 61.54%−330.11%. Compared with T1 in flag leaves, T2 treatment significantly increased SPAD values by 7.90%−23.38%, increased SOD, POD, and CAT activity by 10.63%−32.28%, 14.20%−57.20%, and 10.75%−115.56%, and decreased MDA content by 6.66%−36.08%. And in roots, T2 treatment significantly increased root activity by 44.71%−139.75%, SOD and POD activities by 12.13%−102.01% and 21.05%−146.61%, decreased MDA content by 12.01%−51.23%. The 9 physiological activity indexes were correlated closely with each other, and the ranking of the comprehensive physiological activity index was as follows: YNCK>XMCK>YNT2>XMT2>YNT1>XMT1.
    Conclusions Application of KH2PO4 could alleviate the damage of late spring coldness stress as it could enhance the relative chlorophyll contents of wheat flag leaves, root activity, and antioxidant enzyme activities, reduce MDA content in both flag leaves and roots, thus delaying the aging processes of flag leaves and roots.

     

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